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            • Primary Antibodies ›
            • EED Antibodies

            Invitrogen

            EED Polyclonal Antibody

            Advanced Verification
            3 Published Figures
            3 References
            View all (20) EED antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # PA5-34430
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            Price (USD)
            540.00
            100 µg
             
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            Datasheet
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            Cite EED Polyclonal Antibody

            • Antibody Testing Data (6)
            • Published Figures (3)
            • Advanced Verification (1)
            Application
            EED Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 10

            EED Antibody (PA5-34430) in ICC/IF

            Immunofluorescence analysis of EED was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EED Rabbit Polyclonal Antibody (Product # PA5-34430) at 20 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
            View Product

            EED Antibody in Immunocytochemistry (ICC/IF)
            EED Antibody in Immunohistochemistry (IHC)
            EED Antibody in Western Blot (WB)
            EED Antibody in Western Blot (WB)
            EED Antibody in Western Blot (WB)
            EED Antibody in ChIP Assay (ChIP)
            EED Antibody in Immunohistochemistry (IHC)
            EED Antibody in Immunohistochemistry (IHC)
            EED Antibody in Western Blot, ChIP Assay (WB, ChIP)
            EED Antibody

            Product Details

            PA5-34430

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            2 µg/mL
            View 1 publication 1 publication

            Immunohistochemistry (IHC)

            20 µg/mL
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            20 µg/mL
            -

            ChIP assay (ChIP)

            2.5 µg/10^6 cells
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            An 18 amino acid synthetic peptide near the amino terminus of human EED
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS

            Contains

            0.02% sodium azide

            Storage conditions

            Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2551782

            Product Specific Information

            A suggested positive control is human heart tissue lysate.

            PA5-34430 can be used with blocking peptide PEP-1472.

            Target Information

            The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin Beta-7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Related Products for

            Bioinformatics

            Protein Aliases: Embryonic ectoderm development protein; hEED; lethal, Chr 7, Rinchik 5; Polycomb protein EED; WAIT-1; WD protein associating with integrin cytoplasmic tails 1

            View more View less

            Gene Aliases: EED; ENSMUSG00000039373; HEED; l(7)5Rn; l7Rn5; lusk; WAIT1

            View more View less

            UniProt ID: (Human) O75530, (Mouse) Q921E6

            View more View less

            Entrez Gene ID: (Human) 8726, (Mouse) 13626, (Rat) 293104

            View more View less

            Function(s)

            chromatin binding protein binding histone methyltransferase activity identical protein binding chromatin/chromatin-binding, or -regulatory protein

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter regulation of gene expression by genetic imprinting transcription, DNA-templated histone methylation spinal cord development negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of histone H3-K27 methylation regulation of transcription, DNA-templated chromatin modification
            It has to be done as per old AB suggested Products section.
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