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Application
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EHD1 Antibody (PA5-66950) in ICC/IF
Immunofluorescent staining of EHD1 in human cell line U-2 OS shows localization to plasma membrane. Samples were probed using an EHD1 Polyclonal Antibody (Product # PA5-66950).
Product Details
PA5-66950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human EHD1. Recombinant protein control fragment (Product #RP-105436).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2664757
Product Specific Information
Immunogen sequence: MEQPGTAASPV SGSMFSWVSK DARRKKEPEL FQTVAEGLRQ LYAQKLLP
Highest antigen sequence identity to the following orthologs - mouse 71%, rat 71%.
Target Information
EHD1 acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. EHD1 also plays a role in myoblast fusion and is recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Further, EHD1 belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The EHD1 protein is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for the EHD1 gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EH domain-containing protein 1; EH-domain containing 1; hPAST1; PAST homolog 1; Testilin
Gene Aliases: CDABP0131; EHD1; H-PAST; HPAST1; PAST; PAST1
UniProt ID: (Human) Q9H4M9
Entrez Gene ID: (Human) 10938
Performance Guarantee
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