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Application
FIGURE: 1 / 5
EHD2 Antibody (PA5-18303) in ICC/IF
Immunocytochemistry analysis of EHD2 using EHD2 Polyclonal Antibody (Product # PA5-18303) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18303
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (CRLVPPSKRRHKGSA) corresponding to the C-terminus amino acids of EHD2 (aa 533-543).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10980459
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EH domain-containing protein 2; EH-domain containing 2; FLJ96617; PAST homolog 2
Gene Aliases: EHD2; PAST2
UniProt ID: (Human) Q9NZN4
Entrez Gene ID: (Human) 30846
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