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Invitrogen
EOMES Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
EOMES Antibody (720200) in ICC/IF
Immunofluorescence analysis of EOMES was performed using 70% confluent log phase MCF7 and A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with EOMES Polyclonal Antibody (Product # 720200) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790... View More
Product Details
720200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Protein corresponding to Human TBR2 (aa 456-613)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633200
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Cat, Pig and Dog
Target Information
TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eomesodermin; Eomesodermin homolog; T-box brain protein 2; T-brain-2
Gene Aliases: C77258; EOMES; TBR-2; TBR2
UniProt ID: (Human) O95936, (Mouse) O54839
Entrez Gene ID: (Human) 8320, (Rat) 316052, (Mouse) 13813
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
endoderm formation
mesoderm formation
endodermal cell fate specification
trophectodermal cell differentiation
adaptive immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
transcription, DNA-templated
brain development
cardioblast differentiation
stem cell population maintenance
olfactory bulb development
cerebral cortex regionalization
cerebral cortex neuron differentiation
interferon-gamma production
skeletal muscle cell differentiation
positive regulation of cell differentiation
regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell differentiation involved in embryonic placenta development
mesodermal to mesenchymal transition involved in gastrulation
blastocyst development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
regulation of gene expression
cell differentiation
regulation of neurogenesis
immune system process
regulation of transcription, DNA-templated
multicellular organism development
gastrulation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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