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ERK1/ERK2 Antibody (MA5-50188) in IHC (P)
Immunohistochemistry analysis with a ERK1/ERK2 monoclonal antibody (Product # MA5-50188) using a dilution of 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was i... View More
Product Details
MA5-50188
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
29B10
Immunogen
A synthesized peptide derived from Human MAPK1/MAPK3
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3094378
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2
Gene Aliases: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; Mnk1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3
UniProt ID: (Human) P28482, (Human) P27361, (Mouse) P63085, (Mouse) Q63844
Entrez Gene ID: (Human) 5594, (Human) 5595, (Mouse) 26413, (Mouse) 26417
phosphotyrosine binding
DNA binding
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
transcription factor binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
phosphatase binding
mitogen-activated protein kinase kinase kinase binding
identical protein binding
scaffold protein binding
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
MAPK import into nucleus
transcription, DNA-templated
protein phosphorylation
apoptotic process
chemotaxis
response to stress
cellular response to DNA damage stimulus
cell cycle
signal transduction
chemical synaptic transmission
axon guidance
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
response to toxic substance
positive regulation of peptidyl-threonine phosphorylation
regulation of phosphatidylinositol 3-kinase signaling
viral process
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
sensory perception of pain
cytosine metabolic process
platelet activation
positive regulation of cell migration
thyroid gland development
regulation of protein stability
lipopolysaccharide-mediated signaling pathway
positive regulation of telomere maintenance via telomerase
regulation of stress-activated MAPK cascade
mammary gland epithelial cell proliferation
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB signaling pathway
outer ear morphogenesis
response to exogenous dsRNA
response to estrogen
negative regulation of cell differentiation
positive regulation of translation
positive regulation of transcription, DNA-templated
thymus development
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of sequence-specific DNA binding transcription factor activity
regulation of cytoskeleton organization
positive regulation of telomerase activity
Bergmann glial cell differentiation
long-term synaptic potentiation
face development
JAK-STAT cascade involved in growth hormone signaling pathway
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
cardiac neural crest cell development involved in heart development
ERK1 and ERK2 cascade
response to epidermal growth factor
caveolin-mediated endocytosis
regulation of Golgi inheritance
cellular response to granulocyte macrophage colony-stimulating factor stimulus
regulation of cellular response to heat
positive regulation of telomere capping
regulation of early endosome to late endosome transport
positive regulation of protein phosphorylation
transcription initiation from RNA polymerase I promoter
protein complex assembly
DNA damage induced protein phosphorylation
phosphorylation
arachidonic acid metabolic process
BMP signaling pathway
positive regulation of cyclase activity
positive regulation of histone phosphorylation
positive regulation of histone acetylation
peptidyl-tyrosine autophosphorylation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
positive regulation of ERK1 and ERK2 cascade
interleukin-1-mediated signaling pathway
cellular response to mechanical stimulus
positive regulation of xenophagy
negative regulation of apolipoprotein binding
organ morphogenesis
response to lipopolysaccharide
intracellular signal transduction
cellular response to organic substance
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