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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • IRE1 alpha Antibodies

            Abnova

            ERN1 Monoclonal Antibody (3D1)

            View all (48) IRE1 alpha antibodies
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            Cite ERN1 Monoclonal Antibody (3D1)

            • Antibody Testing Data (3)
            Application
            ERN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            ERN1 Antibody (H00002081-M06) in IHC (P)

            Immunoperoxidase of monoclonal antibody to ERN1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration is 1.5 µg/mL.
            View Product

            ERN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            ERN1 Antibody in Western Blot (WB)
            ERN1 Antibody in ELISA (ELISA)

            Product Details

            H00002081-M06

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1-5 µg/mL
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1.5 µg/mL
            -

            ELISA (ELISA)

            0.3 ng/mL
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Mouse / IgG1, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            3D1

            Immunogen

            ERN1 (AAC25991, 401 a.a. approximately 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            See Label

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.4

            Contains

            no preservative

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Sequence of this protein is as follows: EEVINLVDQT SENAPTTVSR DVEEKPAHAP ARPEAPVDSM LKDMATIILS TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ KELEKIQLLQ QQQQQLPFHP

            Target Information

            ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1

            View more View less

            Gene Aliases: ERN1; hIRE1p; IRE1; IRE1a; IRE1P

            View more View less

            UniProt ID: (Human) O75460

            View more View less

            Entrez Gene ID: (Human) 2081

            View more View less

            Function(s)

            magnesium ion binding endoribonuclease activity protein serine/threonine kinase activity protein binding ATP binding enzyme binding Hsp70 protein binding identical protein binding protein homodimerization activity ADP binding Hsp90 protein binding endoribonuclease

            Process(es)

            endothelial cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated mRNA cleavage mRNA catabolic process protein phosphorylation activation of signaling protein activity involved in unfolded protein response cell cycle arrest activation of JUN kinase activity regulation of macroautophagy positive regulation of RNA splicing response to endoplasmic reticulum stress cellular response to vascular endothelial growth factor stimulus peptidyl-serine autophosphorylation IRE1-mediated unfolded protein response protein autophosphorylation mRNA splicing, via endonucleolytic cleavage and ligation mRNA endonucleolytic cleavage involved in unfolded protein response intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to glucose stimulus RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of endoplasmic reticulum unfolded protein response insulin metabolic process peptidyl-serine trans-autophosphorylation
            It has to be done as per old AB suggested Products section.
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