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ETV4 Antibody (PA5-120384) in WB
Western blot analysis of ETV4 in extracts of various cell lines. Samples were incubated with ETV4 Polyclonal antibody (Product # PA5-120384) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-120384
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-246 of human ETV4 (NP_0019771)
Conjugate
Form
Liquid
Concentration
1.11 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913956
Product Specific Information
Positive test controls include: K-562, Rat brain. The target is usually found in the following locations: Nucleus.
Immunogen sequence: MDPGSLPPLD SEDLFQDLSH FQETWLAEAQ VPDSDEQFVP DFHSENLAFH SPTTRIKKEP QSPRTDPALS CSRKPPLPYH HGEQCLYSSA YDPPRQIAIK SPAPGALGQS PLQPFPRAEQ RNFLRSSGTS QPHPGHGYLG EHSSVFQQPL DICHSFTSQG GGREPLPAPY QHQLSEPCPP YPQQSFKQEY HDPLYEQAGQ PAVDQGG
Target Information
ETV4: ets variant gene 4 (E1A enhancer binding protein, E1AF), also known as PEA3. Several members of the Ets gene family are known to encode sequencespecific DNA binding proteins. These include mouse PU. 1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU. 1, although PU. 1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein, E1AF); ets variant gene 4 (E1A enhancer-binding protein, E1AF); ETS variant protein 4; EWS protein/E1A enhancer binding protein chimera; PEA3 protein; Polyomavirus enhancer activator 3; POLYOMAVIRUS ENHANCER ACTIVATOR 3 (PEA3 PROTEIN) (ETS TRANSLOCATION VARIANT 4); Polyomavirus enhancer activator 3 homolog; polyomavirus enhancer activator-3; Protein PEA3
Gene Aliases: AW414408; E1A-F; E1AF; ETV4; Pea-3; PEA3; PEAS3
UniProt ID: (Human) P43268, (Mouse) P28322
Entrez Gene ID: (Human) 2118, (Rat) 360635, (Mouse) 18612
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
transcription from RNA polymerase II promoter
motor neuron axon guidance
cell differentiation
negative regulation of mammary gland epithelial cell proliferation
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
branching involved in mammary gland duct morphogenesis
regulation of branching involved in mammary gland duct morphogenesis
regulation of transcription from RNA polymerase II promoter
positive regulation of gene expression
positive regulation of transcription, DNA-templated
transcription, DNA-templated
regulation of transcription, DNA-templated
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