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ETV4 Antibody (CF811406) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETV4 (RC202010, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ETV4. (1. Positive lysates LY421534 (100 µg) and LC421534 (20 µg) can be purchased separately from OriGene.
Product Details
CF811406
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1E11
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-276 of human ETV4 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
ETV4: ets variant gene 4 (E1A enhancer binding protein, E1AF), also known as PEA3. Several members of the Ets gene family are known to encode sequencespecific DNA binding proteins. These include mouse PU. 1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU. 1, although PU. 1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein, E1AF); ets variant gene 4 (E1A enhancer-binding protein, E1AF); ETS variant protein 4; EWS protein/E1A enhancer binding protein chimera; PEA3 protein; Polyomavirus enhancer activator 3; POLYOMAVIRUS ENHANCER ACTIVATOR 3 (PEA3 PROTEIN) (ETS TRANSLOCATION VARIANT 4); Polyomavirus enhancer activator 3 homolog; polyomavirus enhancer activator-3; Protein PEA3
Gene Aliases: AW414408; E1A-F; E1AF; ETV4; Pea-3; PEA3; PEAS3
UniProt ID: (Human) P43268, (Mouse) P28322
Entrez Gene ID: (Human) 2118, (Rat) 360635, (Mouse) 18612
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
transcription from RNA polymerase II promoter
motor neuron axon guidance
cell differentiation
negative regulation of mammary gland epithelial cell proliferation
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
branching involved in mammary gland duct morphogenesis
regulation of branching involved in mammary gland duct morphogenesis
regulation of transcription from RNA polymerase II promoter
positive regulation of gene expression
positive regulation of transcription, DNA-templated
transcription, DNA-templated
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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