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Application
FIGURE: 1 / 8
ETV5 Antibody (PA5-30023) in ICC/IF
Immunofluorescence analysis of ETV5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ETV5 Polyclonal Antibody (Product # PA5-30023) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034... View More
Product Details
PA5-30023
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ERM. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.48 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547497
Product Specific Information
Recommended positive controls: ERM-transfected 293T.
Predicted reactivity: Mouse (98%), Rat (95%), Pig (98%), Chicken (88%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ETS variant 5, transcription factor of the ETS family, divergent member of the winged helix-turn-helix superfamily, expressed in brain, placenta and other tissues involved in development. Tissue specificity: Ubiquitous. ERM is expressed in Sertoli cells and spermatogonial stem cells (SSCs). Studies on knockout mice have shown that it is required for SSC self-renewal but not differentiation, thus affected mice exhibit progressive germ cell depletion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ETS translocation variant 5; ets-related molecule; Ets-related protein ERM
Gene Aliases: ERM; ETV5
UniProt ID: (Human) P41161
Entrez Gene ID: (Human) 2119
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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