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Bioss

EXOSC10/PMSCL2 Polyclonal Antibody

View all (19) EXOSC10 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Application

EXOSC10/PMSCL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

EXOSC10/PMSCL2 Antibody (BS-13120R) in IHC (P)

Formalin-fixed and paraffin embedded mouse lymph node labeled with Anti-EXOSC10/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

EXOSC10/PMSCL2 Antibody in Western Blot (WB)

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Product Details

BS-13120R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100-1:500

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human EXOSC10/PMSCL2, amino acids 41-140.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a nuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl-2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl patients. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component ofthe RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: autoantigen PM-SCL; Autoantigen PM/Scl 2; Autoantigen PM/Scl 2 homolog; Exosome complex component 10; exosome component 10; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; PM/Scl-100; Polymyositis/scleroderma autoantigen 100 kDa; Polymyositis/scleroderma autoantigen 2; Polymyositis/scleroderma autoantigen 2 homolog

Gene Aliases: EXOSC10; p2; p3; p4; PM-Scl; PM/Scl-100; PMSCL; PMSCL2; RRP6; Rrp6p

UniProt ID: (Human) Q01780, (Mouse) P56960

Entrez Gene ID: (Human) 5394, (Mouse) 50912, (Rat) 313707

Function(s)

nucleotide binding 3'-5'-exoribonuclease activity exoribonuclease activity protein binding poly(A) RNA binding nucleic acid binding RNA binding catalytic activity nuclease activity exonuclease activity 5'-3' exoribonuclease activity 3'-5' exonuclease activity hydrolase activity

Process(es)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay maturation of 5.8S rRNA nuclear-transcribed mRNA catabolic process rRNA processing dosage compensation by inactivation of X chromosome nuclear mRNA surveillance CUT catabolic process nuclear polyadenylation-dependent rRNA catabolic process histone mRNA catabolic process nuclear retention of unspliced pre-mRNA at the site of transcription RNA phosphodiester bond hydrolysis, exonucleolytic nucleobase-containing compound metabolic process RNA processing cellular metabolic process nucleic acid phosphodiester bond hydrolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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