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Invitrogen
Embigin Monoclonal Antibody (G7.43.1), PE, eBioscience™
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Application
FIGURE: 1 / 1
Embigin Antibody (12-5839-82) in Flow
Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R FITC (Product # 11-0452-82) and 0.5 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (left) or 0.5 µg of Anti-Mouse Embigin PE (right). Total viable cells were used for analysis.
Product Details
12-5839-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
G7.43.1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2016706
Product Specific Information
Description: This G7.43.1 monoclonal antibody reacts with mouse embigin (gp70), a single transmembrane glycoprotein that is a member of the Ig superfamily similar to basigin and EMMPRIN. It contains two Ig domains thought to be involved in cellular adhesion and tissue remodeling. Expression of embigin is high before day 10 in fetal development. In the adult it is expressed on myeloid cells and T cells but not B cells. During hematopoeisis, embigin is expressed on HSCs and CLPs (common lymphoid progenitors) but absent on pro- and pre-B cells. In recent studies Pax 5 has been shown to repress embigin expression.
Applications Reported: This G7.43.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This G7.43.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Embigin is a 327 amino acid regulatory protein belonging to the immunoglobulin superfamily class of CAMs, and has two Ig-like (immunoglobulin-like) V- type domains. In situ hybridization analysis shows that Embigin has specific carbohydrate epitopes and plays an important role in early postimplantation embryogenesis. A highly glycosylated transmembrane glycoprotein, Embigin acts as a regulator of cell/ECM interactions during development and homeostasis of normal adult tissues. Transfection studies suggest its role in promotion of integrin-mediated cell-substratum adhesion. Widely expressed in most of the tissues and embryonal carcinoma cells, prostate and mammary cancer cell lines, it may also help in the classification of human carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Embigin; glycoprotein 70; Teratocarcinoma glycoprotein Gp-70
Gene Aliases: AL022799; Emb; Gp70
UniProt ID: (Mouse) P21995
Entrez Gene ID: (Mouse) 13723
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