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Invitrogen

ErbB2 (HER-2) Monoclonal Antibody (L26)

Advanced Verification

5 References

View all (285) Her-2 (ErbB2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Advanced Verification (2)

Application

ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

ErbB2 (HER-2) Antibody (MA5-13679) in ICC/IF

Immunofluorescence analysis of Her2 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Her2 Mouse monoclonal Antibody (Product # MA5-13679) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Cel... View More

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Product Details

MA5-13679

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mg protein lysate

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

L26

Immunogen

Extracellular domain of recombinant human c-erbB-2/HER-2 oncoprotein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983761

Product Specific Information

MA5-13679 targets HER-2 in immunoprecipitation applications and shows reactivity with Human samples.

The MA5-13679 immunogen is extracellular domain of recombinant human c-erbB-2/HER-2 oncoprotein.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BCL6 positively regulates AID and germinal center gene expression via repression of miR-155.

The Journal of experimental medicine

Basso K,Schneider C,Shen Q,Holmes AB,Setty M,Leslie C,Dalla-Favera R

MA5-13679 was used in immunohistochemistry to study the role of miR-155 repression in the mechanism by which BCL6 regulates the expression of AID and germinal centre genes

Mon Dec 17 00:00:00 UTC 2012

Association between growth factor heregulin1α and receptors in growth of ovarian cancer cell line with high potentiality of peritoneal dissemination.

Fukushima journal of medical science

Nishiyama H,Soeda S,Watanabe T,Fujimori K

MA5-13679 was used in immunocytochemistry to study the expression of heregulin-1alpha and it receptors and their association with the growth of an ovarian cancer cell line with high peritoneal dissemination capacity

Wed Jul 24 00:00:00 UTC 2013

Lymphoepithelioma-like carcinoma of the ovary.

International journal of surgical pathology

Ambrosio MR,Rocca BJ,Onorati M,Mourmouras V,Mastrogiulio MG,Crispino S,Liberatore C,Santopietro R

MA5-13679 was used in immunohistochemistry to report on a case of lymphoepithelioma-like carcinoma of the ovary

Mon Aug 01 00:00:00 UTC 2011

Identification of a morphometrical parameter that predicts the response to splenectomy in patients with idiopathic thrombocytopenic purpura.

The Tohoku journal of experimental medicine

Bakkaloglu H,Dinccag A,Yanar H,Tunca F,Dogan O,Cermik H,Kucukkaya R

MA5-13679 was used in immunohistochemistry to study mean maximun follicle diameter as a parameter for predicting the outcome of splenectomy in idiopathic thrombocytopenic purpura patients

Fri Sep 01 00:00:00 UTC 2006

A chimeric multi-human epidermal growth factor receptor-2 B cell epitope peptide vaccine mediates superior antitumor responses.

Journal of immunology (Baltimore, Md. : 1950)

Dakappagari NK,Pyles J,Parihar R,Carson WE,Young DC,Kaumaya PT

MA5-13679 was used in blocking or activating experiment to develop and evaluate a multi-epitope vaccine targeting HER-2

Tue Apr 15 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: c-erb B2/neu protein; CD antigen CD340; CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

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Gene Aliases: CD340; ERBB2; HER-2; HER-2/neu; HER2; MLN 19; MLN19; NEU; NGL; TKR1

UniProt ID: (Human) P04626

Entrez Gene ID: (Human) 2064

Function(s)

RNA polymerase I core binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding protein C-terminus binding growth factor binding protein phosphatase binding identical protein binding ErbB-3 class receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity protein dimerization activity transmembrane signal receptor

Process(es)

Performance Guarantee

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