Invitrogen

ErbB3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (83) Her-3 (ErbB3) antibodies
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Datasheet
Protocols
Questions & Answers
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Product Details

PA5-14636

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1:10-1:50
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human ERBB3
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2099571

Target Information

ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: avian erythroblastosis oncogene B 3 receptor; c-erbB-3; C-errB-3; Glial growth factor receptor; human epidermal growth factor receptor 3; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3

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Gene Aliases: c-erbB-3; c-erbB3; C76256; ErbB-3; ERBB3; erbB3-S; Erbb3r; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3

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UniProt ID: (Human) P21860, (Mouse) Q61526

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Entrez Gene ID: (Human) 2065, (Mouse) 13867

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Function(s)
protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding growth factor binding protein tyrosine kinase activator activity neuregulin binding identical protein binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups neuregulin receptor activity transmembrane signal receptor
Process(es)
MAPK cascade endocardial cushion development negative regulation of cell adhesion signal transduction transmembrane receptor protein tyrosine kinase signaling pathway peripheral nervous system development heart development negative regulation of signal transduction Schwann cell differentiation phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cranial nerve development ERBB2 signaling pathway wound healing regulation of cell proliferation negative regulation of neuron apoptotic process positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling negative regulation of secretion neuron apoptotic process positive regulation of protein tyrosine kinase activity positive regulation of calcineurin-NFAT signaling cascade extrinsic apoptotic signaling pathway in absence of ligand regulation of cell motility protein phosphorylation epidermal growth factor receptor signaling pathway phosphorylation positive regulation of glucose import
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