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Rockland
Erpd/Arp37 Polyclonal Antibody
Application
FIGURE: 1 / 1
Erpd/Arp37 Antibody (200-401-C09) in WB
Western Blot showing detection of 0.1 µg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpD/Arp37 fusion proteins (arrow: expected MW of major band: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocki... View More
Product Details
200-401-C09
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi ErpD/Arp37 protein.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
It is directed against, and shows specific reactivity for, Borrelia burgdorferi ErpD protein. Reactivity with ErpD/Arp37 protein from other sources has not been determined.
Target Information
Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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