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            • Primary Antibodies ›
            • Estrogen Receptor alpha Antibodies

            Invitrogen

            Estrogen Receptor alpha Monoclonal Antibody (SP1)

            Advanced Verification
            10 Published Figures
            286 References
            View all (90) Estrogen Receptor alpha antibodies
            Datasheet
            Protocols
            Questions & Answers
            Tech Support
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            Catalog # MA5-14501
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            Price (HKD)
            8,566.00
            500 µL
             
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            Datasheet
            Protocols
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            Cite Estrogen Receptor alpha Monoclonal Antibody (SP1)

            • Antibody Testing Data (7)
            • Published Figures (10)
            • Advanced Verification (3)
            Application
            Estrogen Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 20

            Estrogen Receptor alpha Antibody (MA5-14501) in ICC/IF

            Immunofluorescence analysis of Estrogen Receptor alpha was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with beta Estrogen Receptor alpha Monoclonal Antibody (Product # MA5-14501) at 1:200 dilution in 0.... View More
            View Product

            Estrogen Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
            Estrogen Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
            Estrogen Receptor alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
            Estrogen Receptor alpha Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor alpha Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor alpha Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor alpha Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor alpha Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody in Western Blot (WB)
            Estrogen Receptor alpha Antibody
            Estrogen Receptor alpha Antibody
            Estrogen Receptor alpha Antibody

            Product Details

            MA5-14501

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            View 31 publications 31 publications

            Immunohistochemistry (IHC)

            -
            View 213 publications 213 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:200
            View 27 publications 27 publications

            Immunocytochemistry (ICC/IF)

            1:25-1:250
            View 10 publications 10 publications

            Flow Cytometry (Flow)

            1:200
            View 1 publication 1 publication

            Dot blot (DB)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 3 publications 3 publications
            Product Specifications

            Species Reactivity

            Human

            Published species

            Human, Mouse, Rat, Sheep, Virus

            Host/Isotype

            Rabbit / IgG

            Class

            Monoclonal

            Type

            Antibody

            Clone

            SP1

            Immunogen

            Synthetic peptide derived from C-terminal of human estrogen receptor
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.015 mg/mL

            Purification

            Protein A

            Storage buffer

            PBS, pH 7.2, with 1% BSA

            Contains

            0.1% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_10981779

            Product Specific Information

            This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. It reacts with ER alpha, 67 kDa. No special pretreatment is required for IHC staining.

            Target Information

            Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Related Products for

            Bioinformatics

            Protein Aliases: DKFZp686N23123; ER; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

            View more View less

            Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1

            View more View less

            UniProt ID: (Human) P03372

            View more View less

            Entrez Gene ID: (Human) 2099

            View more View less

            Function(s)

            RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter antral ovarian follicle growth osteoblast development chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration androgen metabolic process negative regulation of gene expression positive regulation of phospholipase C activity intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to estradiol negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of sequence-specific DNA binding transcription factor activity regulation of neuron apoptotic process response to estrogen positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import decidualization positive regulation of fibroblast proliferation negative regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell development Sertoli cell proliferation uterus development vagina development prostate epithelial cord elongation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis mammary gland branching involved in pregnancy mammary gland alveolus development epithelial cell proliferation involved in mammary gland duct elongation positive regulation of ERK1 and ERK2 cascade protein localization to chromatin cellular response to estrogen stimulus cellular response to estradiol stimulus negative regulation of triglyceride metabolic process negative regulation of neuron death negative regulation of production of miRNAs involved in gene silencing by miRNA baculum development
            It has to be done as per old AB suggested Products section.
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