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Bioss

F-Actin Polyclonal Antibody

View all (2) F-actin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Application

F-Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

F-Actin Antibody (BS-1571R) in IHC (P)

Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.

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Product Details

BS-1571R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:20-1:100

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit, amino acids 1-50.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; Atcb; Bact; actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoplasmic beta-actin; PS1TP5-binding protein 1

Gene Aliases: ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

UniProt ID: (Human) P60709, (Chicken) P60706, (Dog) O18840, (Rat) P60711, (Mouse) P60710

Entrez Gene ID: (Human) 60, (Chicken) 396526, (Dog) 403580, (Rat) 81822, (Mouse) 11461

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding structural constituent of postsynaptic actin cytoskeleton actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis cell motility postsynaptic actin cytoskeleton organization retina development in camera-type eye cellular response to electrical stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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