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Invitrogen

F-actin Monoclonal Antibody (NH3)

5 References

View all (2) F-actin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Product Details

MA1-80729

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

1:10

ELISA (ELISA)

1:10

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

NH3

Immunogen

Human monocytes and U937 cell line.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_936561

Product Specific Information

Mouse anti Human F-Actin antibody, clone NH3 recognizes human Filamentous actin (F-actin).

Target Information

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KISS1R and ANKRD31 Cooperate to Enhance Leydig Cell Gene Expression via the Cytoskeletal-Nucleoskeletal Pathway.

Frontiers in cell and developmental biology

Ricci G,Guillou F,Catizone A,Mele VG,Moggio M,Chioccarelli T,Diano N,Meccariello R,Pierantoni R,Fasano S,Cobellis G,Chianese R,Manfrevola F

MA1-80729 was used in Western Blot, Immunocytochemistry to highlight a novel role for the Kisspeptin-10 in the induction of the cytoskeletal-nucleoskeletal route, downstream a physical interaction between KISS1 receptor and ANKRD31, with gene expression activation as final effect, in Leydig cells.

Sat Jul 16 00:00:00 UTC 2022

KISS1R and ANKRD31 Cooperate to Enhance Leydig Cell Gene Expression via the Cytoskeletal-Nucleoskeletal Pathway.

Frontiers in cell and developmental biology

Ricci G,Guillou F,Catizone A,Mele VG,Moggio M,Chioccarelli T,Diano N,Meccariello R,Pierantoni R,Fasano S,Cobellis G,Chianese R,Manfrevola F

Sat Jul 16 00:00:00 UTC 2022

The Role of Cytoskeletal Proteins in the Formation of a Functional In Vitro Blood-Brain Barrier Model.

International journal of molecular sciences

Mentor S,Makhathini KB,Fisher D

MA1-80729 was used in Immunocytochemistry to show that the identification of specific cytoskeletal structures in TENTs endorsed the postulate of their indispensable role in barrier-genesis and the maintenance of regulatory permeability across the BBB.

Tue Jan 11 00:00:00 UTC 2022

Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis.

Frontiers in cell and developmental biology

Roversi FM,Bueno MLP,Pericole FV,Saad STO

MA1-80729 was used in Immunocytochemistry to study the role of one SFK member, the Hematopoietic Cell Kinase (HCK), in CXCL12/CXCR4 pathway and its contribution to the AML pathogenesis.

Tue Apr 13 00:00:00 UTC 2021

Sequential targeting of YAP1 and p21 enhances the elimination of senescent cells induced by the BET inhibitor JQ1.

Cell death & disease

Zhang HT,Gui T,Liu RX,Tong KL,Wu CJ,Li Z,Huang X,Xu QT,Yang J,Tang W,Sang Y,Liu W,Liu N,Ross RD,He QY,Zha ZG

MA1-80729 was used in Immunocytochemistry-immunoflourescence to study whether YAP1 expression was upregulated in CHS tissues, and if it positively correlated with its grading score.

Mon Jan 25 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; Atcb; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1

Gene Aliases: ACT-5; ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

UniProt ID: (Rabbit) P29751, (Human) P60709, (Rat) P60711, (Mouse) P60710

Entrez Gene ID: (Rabbit) 100009272, (Human) 60, (Rat) 81822, (Mouse) 11461

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis retina development in camera-type eye cellular response to electrical stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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F-actin Monoclonal Antibody (NH3)

Product Details

MA1-80729

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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