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Application
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F4/80 Antibody (MA5-28197) in Flow
Flow cytometry of EMR1 in human eosinophils. Sample was incubated with EMR1 monoclonal antibody (Product # MA5-28197), followed by Goat anti Hamster IgG - Biotin and Streptavidin - RPE.
Product Details
MA5-28197
Product Specifications
Species Reactivity
Human
Host/Isotype
Hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
A10
Immunogen
Fibroblast cell line ARH102 expressing CD97-EMR1 (EGF1-6)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Purification
Protein G
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745168
Product Specific Information
This antibody recognizes human EMR1 (EGF-like module containing, mucin-like, hormone receptor-like 1). EMR1 is a seven-span transmembrane protein which is a member of the EGF-TM7 family of receptors. The detailed structure and function of EMR1 is still unknown.
Reconstitute with 1.0 mL distilled water
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adhesion G protein-coupled receptor E1; egf-like module containing, mucin-like, hormone receptor-like 1; egf-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor
Gene Aliases: ADGRE1; EMR1; TM7LN3
UniProt ID: (Human) Q14246
Entrez Gene ID: (Human) 2015
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