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            • Primary Antibodies ›
            • FAK Antibodies

            Invitrogen

            FAK Polyclonal Antibody

            Advanced Verification
            40 Published Figures
            43 References
            View all (105) FAK antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # AHO0502
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            Price (USD)
            409.65
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            546.00
            Save 136.35 (25%)
            500 µg
             
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            Datasheet
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            Questions & Answers

            Cite FAK Polyclonal Antibody

            • Antibody Testing Data (2)
            • Published Figures (40)
            • Advanced Verification (3)
            Application
            FAK Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 45

            FAK Antibody (AHO0502) in WB

            CRISPR-Cas9 mediated genome editing ofFAK (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR627416_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of FAK was performed by loading 30 µg of A549 wild type (Lane 1), A549 Cas9 (Lane 2) and A549 Cas9 cells transduced with FAK Lentiviral sgRNA (Lane 3) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel (Product... View More
            View Product

            FAK Antibody in Western Blot (WB)
            FAK Antibody
            FAK Antibody in Immunocytochemistry (ICC/IF)
            FAK Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            FAK Antibody in Immunocytochemistry (ICC/IF)
            FAK Antibody in Immunocytochemistry (ICC/IF)
            FAK Antibody in Immunocytochemistry (ICC/IF)
            FAK Antibody in Immunocytochemistry (ICC/IF)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot, Immunoprecipitation (WB, IP)
            FAK Antibody in Western Blot, Immunoprecipitation (WB, IP)
            FAK Antibody in Western Blot, Immunoprecipitation (WB, IP)
            FAK Antibody in Western Blot, Immunoprecipitation (WB, IP)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot, Immunoprecipitation (WB, IP)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot, Immunoprecipitation (WB, IP)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody in Western Blot (WB)
            FAK Antibody
            FAK Antibody
            FAK Antibody

            Product Details

            AHO0502

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            Assay-dependent
            View 31 publications 31 publications

            Immunocytochemistry (ICC/IF)

            -
            View 7 publications 7 publications

            Immunoprecipitation (IP)

            Assay-dependent
            View 4 publications 4 publications

            Functional Assay (Functional)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Published species

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Recombinant protein corresponding to amino acid residues 853-1052 from theC-terminal region of human FAK.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Protein A

            Storage buffer

            PBS, pH 7.3, with 0.2% BSA

            Contains

            0.1% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2536313

            Target Information

            Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Related Products for

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            • Secondary Antibodies
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            • Labeling Kits WB

            Bioinformatics

            Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

            View more View less

            Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

            View more View less

            UniProt ID: (Human) Q05397, (Mouse) P34152, (Rat) O35346

            View more View less

            Entrez Gene ID: (Human) 5747, (Mouse) 14083, (Rat) 25614

            View more View less

            Function(s)

            actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding identical protein binding phosphatidylinositol 3-kinase binding

            Process(es)

            MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development heart morphogenesis cellular component disassembly involved in execution phase of apoptosis axon guidance embryo development regulation of endothelial cell migration establishment of cell polarity netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity vascular endothelial growth factor receptor signaling pathway cell motility regulation of cytoskeleton organization cytoskeleton organization response to mechanical stimulus response to glucose response to organic substance response to organonitrogen compound response to organic cyclic compound response to estradiol response to drug fat cell differentiation response to arsenic-containing substance
            It has to be done as per old AB suggested Products section.
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