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Invitrogen
FANCD2 Polyclonal Antibody
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Application
FIGURE: 1 / 11
FANCD2 Antibody (PA1-16548) in ICC/IF
Immunofluorescence analysis of FANCD2 was performed using 70% confluent log phase HeLa cells treated with 2mM of Hydroxyurea for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FANCD2 Rabbit Polyclonal Antibody (Product # PA1-16548) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibo... View More
Product Details
PA1-16548
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Dog,
Human,
Marsupial,
Mouse,
Non-human primate,
Rat,
Zebrafish
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Human FANCD2 fusion protein (N-terminal fragment).
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2102037
Product Specific Information
In Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form).
Additional bands may be seen at lower molecular weights.
In immunofluorescence, this has been tested in human MMC and IR-treated MEF cells.Suggested positive control: Hela whole cell extract.
Target Information
FANCD2 (Fanconi anemia group D2 protein) is required for maintenance of chromosomal stability. It promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-stranded breaks, both by homologous recombination and single-stranded annealing. It is required for the targeting/stabilization of BLM to non-centromeric abnormal structures induced by replicative stress. FANCD2 promotes BRCA2/FANCD1 loading onto damaged chromatin. Defects in the FANCD2 gene result in Fanconi anemia complementation group D2. This disorder affects all bone marrow elements resulting in anemia, leukopenia, and thrombopenia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp762A223; FACD2; Fancd2; Fanconi anemia complementation group D2; Fanconi anemia D2 protein; Fanconi anemia group D2 protein; Fanconi anemia group D2 protein homolog; Fanconi anemia, complementation group D2; FLJ23826; Protein FACD2
Gene Aliases: 2410150O07Rik; AU015151; BB137857; FA-D2; FA4; FACD; FAD; FAD2; FANCD; FANCD2
UniProt ID: (Human) Q9BXW9, (Mouse) Q80V62, (Rat) Q6IV68
Entrez Gene ID: (Dog) 484659, (Human) 2177, (Mouse) 211651, (Rat) 312641, (Zebrafish) 394241
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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