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Invitrogen
FBXW7 Polyclonal Antibody
Application
FIGURE: 1 / 8
FBXW7 Antibody (40-1500) in IHC (P)
Immunohistochemistry analysis of Cdc4 showing staining in the cytoplasm and nucleus of paraffin-embedded human cervical carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min ... View More
Product Details
40-1500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from an internal region of the human and mouse Cdc4
Conjugate
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533451
Target Information
F-Box and WD Repeat Domain Containing 7 (FBXW7) is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXW7 was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: archipelago; Archipelago homolog; DKFZp686F23254; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila); F-box and WD-40 domain protein 7, archipelago homolog; F-box and WD-40 domain-containing protein 7; f-box only protein 30; F-box protein FBW7; F-box protein FBX30; F-box protein Fbxw6; F-box protein SEL-10; F-box-WD40 repeat protein 6; F-box/WD repeat-containing protein 7; FLJ11071; FLJ16457; hAgo; hCdc4; homolog of C elegans sel-10; SEL-10
Gene Aliases: 1110001A17Rik; AGO; CDC4; FBW6; FBW7; Fbwd6; FBX30; FBXO30; FBXW6; FBXW7; hAgo; hCdc4; SEL-10; SEL10
UniProt ID: (Human) Q969H0, (Mouse) Q8VBV4
Entrez Gene ID: (Human) 55294, (Mouse) 50754
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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