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Invitrogen
FDC Monoclonal Antibody (CNA.42), eBioscience™
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Application
FIGURE: 1 / 1
FDC Antibody (14-9968-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil using 1 µg/mL Mouse IgM Isotype Control (left) or 1 µg/mL Anti-Human FDC Purified (right) followed by anti-Mouse IgM Biotin and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
14-9968-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
CNA.42
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1311219
Product Specific Information
Description: The monoclonal antibody CNA.42 recognizes a unique carbohydrate epitope found on follicular dendritic cells (FDCs) from a variety of species. FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis. Staining with this CNA.42 monoclonal antibody is mainly restricted to the plasma membrane but in limited cases may also be cytoplasmic (as seen in mast cells).
This monoclonal has been shown to be a useful reagent for both flow cytometry and IHC analysis.
Applications Reported: This CNA.42 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemistry on frozen and formalin-fixed paraffin embedded tissue sections.
Applications Tested: This CNA.42 antibody has been tested by flow cytometric of a human cell line and western blotting. This can be used at less than or equal to 0.5 µg per million cells in a 100 µL total staining volume or 2 µg/mL (for western blotting). The antibody has also been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil using high pH antigen antigen retrieval. For IHC applications, the antibody can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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