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Invitrogen

FGF8 Polyclonal Antibody

View all (17) FGF8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

FGF8 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

FGF8 Antibody (PA5-114734) in ICC/IF

Immunocytochemistry analysis of FGF8 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114734) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

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Product Details

PA5-114734

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human FGF8(Accession P55075), corresponding to amino acid residues L152-T202.

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Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2899370

Product Specific Information

Antibody detects endogenous levels of total FGF8.

Target Information

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Plays a role in neurite outgrowth in hippocampal cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AIGF; androgen-induced; Androgen-induced growth factor; FGF; FGF-8; Fibroblast growth factor; Fibroblast growth factor 8; fibroblast growth factor 8 (androgen-induced); HBGF-8; Heparin-binding growth factor 8; MGC149376

Gene Aliases: AIGF; FGF-8; FGF8; HBGF-8; HH6; KAL6

UniProt ID: (Human) P55075, (Mouse) P37237

Entrez Gene ID: (Human) 2253, (Rat) 29349, (Mouse) 14179

Function(s)

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding growth factor activity 1-phosphatidylinositol-3-kinase activity chemoattractant activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity type 1 fibroblast growth factor receptor binding type 2 fibroblast growth factor receptor binding receptor binding growth factor intercellular signal molecule

Process(es)

MAPK cascade patterning of blood vessels metanephros development branching involved in ureteric bud morphogenesis organ induction mesonephros development neural plate morphogenesis heart looping blood vessel remodeling outflow tract septum morphogenesis epithelial to mesenchymal transition involved in endocardial cushion formation apoptotic process response to oxidative stress gastrulation motor neuron axon guidance mesodermal cell migration positive regulation of cell proliferation gonad development fibroblast growth factor receptor signaling pathway anatomical structure morphogenesis positive regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling response to organic cyclic compound peptidyl-tyrosine phosphorylation pallium development subpallium development forebrain dorsal/ventral pattern formation cell proliferation in forebrain forebrain neuron development signal transduction involved in regulation of gene expression BMP signaling pathway male genitalia development thyroid gland development otic vesicle formation midbrain-hindbrain boundary development dorsal/ventral axon guidance embryonic hindlimb morphogenesis aorta morphogenesis phosphatidylinositol-3-phosphate biosynthetic process odontogenesis regulation of odontogenesis of dentin-containing tooth response to drug negative regulation of neuron apoptotic process positive regulation of GTPase activity cell fate commitment positive regulation of mitotic nuclear division positive regulation of organ growth phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling forebrain morphogenesis positive chemotaxis positive regulation of cell division negative regulation of cardiac muscle tissue development pharyngeal system development canonical Wnt signaling pathway corticotropin hormone secreting cell differentiation thyroid-stimulating hormone-secreting cell differentiation bone development lung morphogenesis branching involved in salivary gland morphogenesis neuroepithelial cell differentiation positive regulation of ERK1 and ERK2 cascade dopaminergic neuron differentiation cell migration involved in mesendoderm migration kidney development heart morphogenesis outflow tract morphogenesis determination of left/right symmetry heart development embryo development ending in birth or egg hatching telencephalon development central nervous system neuron development lung development embryonic heart tube development limb morphogenesis inner ear morphogenesis negative regulation of apoptotic process generation of neurons multicellular organism development cell differentiation

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