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Proteintech

FHL2 Polyclonal Antibody

View all (19) FHL2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (13)

Application

FHL2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

FHL2 Antibody (21619-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

FHL2 Antibody in Immunocytochemistry (ICC/IF)

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FHL2 Antibody in Western Blot (WB)

FHL2 Antibody in Western Blot (WB)

FHL2 Antibody in Western Blot (WB)

FHL2 Antibody in Western Blot (WB)

FHL2 Antibody in Western Blot (WB)

FHL2 Antibody in Western Blot (WB)

FHL2 Antibody in Western Blot (WB)

FHL2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

FHL2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:100 (under 10x lens).

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:100 (under 40x lens).

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:50 (under 40x lens).

FHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21619-1-AP (FHL2 antibody) at dilution of 1:50 (under 10x lens).

FHL2 Antibody in Western Blot (WB) — Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FHL2 Antibody in Western Blot (WB) — Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FHL2 Antibody in Western Blot (WB) — Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FHL2 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FHL2 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FHL2 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FHL2 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FHL2 Antibody in Immunoprecipitation (IP) — IP result of anti-FHL2 (IP:21619-1-AP, 4ug; Detection:21619-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

Product Details

21619-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FHL2 Fusion Protein Ag1378 (1-279 aa encoded by BC014397)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTERFDCHH CNESLFGKKY ILREESPYCV VCFETLFANT CEECGKPIGC DCKDLSYKDR HWHEACFHCS QCRNSLVDKP FAAKEDQLLC TDCYSNEYSS KCQECKKTIM PGTRKMEYKG SSWHETCFIC HRCQQPIGTK SFIPKDNQNF CVPCYEKQHA MQCVQCKKPI TTGGVTYREQ PWHKECFVCT ACRKQLSGQR FTARDDFAYC LNCFCDLYAK KCAGCTNPIS GLGGTKYISF EERQWHNDCF NCKKCSLSLV GRGFLTERDD ILCPDCGKDI (1-279 aa encoded by BC014397)

Target Information

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells.

Cell death & disease

Wang C,Sun H,Davis JS,Wang X,Huo L,Sun N,Huang Q,Lv X,Wang C,He C,He C,Zhou Y,Wu J,Yang L,Hua G

Wed Apr 05 00:00:00 UTC 2023

Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice.

iScience

Ren L,Du W,Song D,Lu H,Hamblin MH,Wang C,Du C,Fan GC,Becker RC,Fan Y

Fri Jan 20 00:00:00 UTC 2023

Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice.

iScience

Ren L,Du W,Song D,Lu H,Hamblin MH,Wang C,Du C,Fan GC,Becker RC,Fan Y

Fri Jan 20 00:00:00 UTC 2023

The mechanical regulation of RNA binding protein hnRNPC in the failing heart.

Science translational medicine

Martino F,Varadarajan NM,Perestrelo AR,Hejret V,Durikova H,Vukic D,Horvath V,Cavalieri F,Caruso F,Albihlal WS,Gerber AP,O'Connell MA,Vanacova S,Pagliari S,Forte G

Wed Nov 23 00:00:00 UTC 2022

The mechanical regulation of RNA binding protein hnRNPC in the failing heart.

Science translational medicine

Martino F,Varadarajan NM,Perestrelo AR,Hejret V,Durikova H,Vukic D,Horvath V,Cavalieri F,Caruso F,Albihlal WS,Gerber AP,O'Connell MA,Vanacova S,Pagliari S,Forte G

Wed Nov 23 00:00:00 UTC 2022

Elevated TAB182 enhances the radioresistance of esophageal squamous cell carcinoma through G2-M checkpoint modulation.

Cancer medicine

Cao Y,Gao A,Li X,Min H,He C,Sun X,Ding WQ,Zhou J

Sat May 01 00:00:00 UTC 2021

Elevated TAB182 enhances the radioresistance of esophageal squamous cell carcinoma through G2-M checkpoint modulation.

Cancer medicine

Cao Y,Gao A,Li X,Min H,He C,Sun X,Ding WQ,Zhou J

Sat May 01 00:00:00 UTC 2021

Oxymatrine Synergistically Enhances Doxorubicin Anticancer Effects in Colorectal Cancer.

Frontiers in pharmacology

Pan D,Zhang W,Zhang N,Xu Y,Chen Y,Peng J,Chen Y,Zhang Y,Shen X

Tue Jul 27 00:00:00 UTC 2021

Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.

EBioMedicine

Zhou R,Li S,Liu J,Wu H,Yao G,Sun Y,Chen ZJ,Li W,Du Y

Sat Feb 01 00:00:00 UTC 2020

Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.

EBioMedicine

Zhou R,Li S,Liu J,Wu H,Yao G,Sun Y,Chen ZJ,Li W,Du Y

Sat Feb 01 00:00:00 UTC 2020

Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.

EBioMedicine

Zhou R,Li S,Liu J,Wu H,Yao G,Sun Y,Chen ZJ,Li W,Du Y

Sat Feb 01 00:00:00 UTC 2020

Expression, location and biological effects of four and a half LIM domain protein 2 (FHL2) on granulosa cells in ovine.

Reproduction in domestic animals = Zuchthygiene

Zhang L,Li R,Chen L,Xing Z,Song Y,Nie X,Wang L,Han H,Liu A,Ma X,Ma RZ,Tian S

Mon Jun 01 00:00:00 UTC 2020

Expression, location and biological effects of four and a half LIM domain protein 2 (FHL2) on granulosa cells in ovine.

Reproduction in domestic animals = Zuchthygiene

Zhang L,Li R,Chen L,Xing Z,Song Y,Nie X,Wang L,Han H,Liu A,Ma X,Ma RZ,Tian S

Mon Jun 01 00:00:00 UTC 2020

Expression, location and biological effects of four and a half LIM domain protein 2 (FHL2) on granulosa cells in ovine.

Reproduction in domestic animals = Zuchthygiene

Zhang L,Li R,Chen L,Xing Z,Song Y,Nie X,Wang L,Han H,Liu A,Ma X,Ma RZ,Tian S

Mon Jun 01 00:00:00 UTC 2020

Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.

Journal of virology

Lukash T,Agback T,Dominguez F,Shiliaev N,Meshram C,Frolova EI,Agback P,Frolov I

Wed Dec 09 00:00:00 UTC 2020

Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.

Journal of virology

Lukash T,Agback T,Dominguez F,Shiliaev N,Meshram C,Frolova EI,Agback P,Frolov I

Wed Dec 09 00:00:00 UTC 2020

B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters.

Infection and immunity

Johnson RM,Yu H,Strank NO,Karunakaran K,Zhu Y,Brunham RC

Thu Feb 01 00:00:00 UTC 2018

B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters.

Infection and immunity

Johnson RM,Yu H,Strank NO,Karunakaran K,Zhu Y,Brunham RC

Thu Feb 01 00:00:00 UTC 2018

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 UTC 2017

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; FHL-2; Four and a half LIM domains protein 2; LIM domain protein DRAL; OTTHUMP00000203164; OTTHUMP00000203168; OTTHUMP00000203169; Skeletal muscle LIM-protein 3; SLIM 3; SLIM-3

Gene Aliases: AAG11; DRAL; FHL-2; FHL2; SLIM-3; SLIM3

UniProt ID: (Human) Q14192, (Mouse) O70433, (Rat) O35115

Entrez Gene ID: (Human) 2274, (Mouse) 14200, (Rat) 63839

Function(s)

transcription coactivator activity protein binding transcription factor binding zinc ion binding identical protein binding androgen receptor binding metal ion binding transcription cofactor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation transcription, DNA-templated response to hormone androgen receptor signaling pathway negative regulation of apoptotic process positive regulation of transcription, DNA-templated atrial cardiac muscle cell development ventricular cardiac muscle cell development heart trabecula formation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter negative regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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