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Invitrogen
FITC/5-FAM/6-FAM Recombinant Rabbit Monoclonal Antibody (4L8R5)
Application
FIGURE: 1 / 2
FITC/5-FAM/6-FAM Antibody (MA5-55271) in WB
FITC/5-FAM/6-FAM Recombinant Monoclonal Antibody (Product # MA5-55271) was tested in Western blot against FITC Goat IgG, TRITC Goat IgG, and Goat IgG at 1:4,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-55271
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4L8R5
Immunogen
FITC
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667513
Product Specific Information
Immunogen Sequence: FITC conjugated to KLH
Target Information
FITC (Fluorescein Isothiocyanate), 5-FAM (5-Carboxyfluorescein), and 6-FAM (6-Carboxyfluorescein) are commonly used gene targets for fluorescent labeling in molecular biology research. These dyes belong to the fluorescein family and emit a green fluorescence when excited by specific wavelengths of light. FITC, 5-FAM, and 6-FAM are utilized in various techniques such as fluorescence in situ hybridization (FISH), flow cytometry, immunofluorescence, and DNA sequencing. By attaching these dyes to gene-specific probes or primers, researchers can visualize and detect gene expression levels, identify specific DNA or RNA sequences, and study cellular processes. The compatibility of FITC, 5-FAM, and 6-FAM with different imaging systems and their bright green fluorescence make them valuable tools in molecular biology, aiding in the understanding of gene function and cellular dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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