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Invitrogen

FMRP Monoclonal Antibody (4G9)

Advanced Verification

1 Published Figure

3 References

View all (32) FMRP antibodies

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Catalog # MA5-15499

Price (KRW)

645,000

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Ends: 30-Jun-2025

758,000

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100 µL

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Datasheet

Protocols

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Application

FMRP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

FMRP Antibody (MA5-15499) in ICC/IF

Immunofluorescence analysis of FMRP was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FMRP Mouse Monoclonal Antibody (Product # MA5-15499) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

FMRP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FMRP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SHSY5Y cells were transfected with FMRP siRNA and reduction of signal was observed in Western Blot using FMRP Monoclonal Antibody (Product # MA5-15499). {KD}

Product Details

MA5-15499

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4G9

Immunogen

Purified recombinant fragment of human FMR1 expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10979450

Product Specific Information

MA5-15499 targets FMR1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15499 immunogen is purified recombinant fragment of human FMR1 expressed in E. Coli.

MA5-15499 detects FMR1 which has a predicted molecular weight of approximately 71kDa.

Target Information

FMR1 binds RNA and is associated with polysomes. The protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure. Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression of FMRpolyG in Peripheral Blood Mononuclear Cells of Women with Fragile X Mental Retardation 1 Gene Premutation.

Genes

Nguyen XP,Vilkaite A,Messmer B,Dietrich JE,Hinderhofer K,Schäkel K,Strowitzki T,Rehnitz J

MA5-15499 was used in Western Blotting to investigate Fragile X-associated primary ovarian insufficiency (FXPOI), revealing the presence of FMRpolyG aggregates in peripheral blood monocyte cells (PBMCs) and granulosa cells (GCs) of FMR1 premutation (PM) carriers,.

Tue Mar 01 00:00:00 UTC 2022

New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules.

Journal of cell science

Das R,Schwintzer L,Vinopal S,Aguado Roca E,Sylvester M,Oprisoreanu AM,Schoch S,Bradke F,Broemer M

Published figure using FMRP monoclonal antibody (Product # MA5-15499) in Immunocytochemistry

Mon Jun 17 00:00:00 UTC 2019

The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion.

eLife

Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

MA5-15499 was used in Western Blotting to show that identifying PTPRK substrates provides insight into its downstream signaling and a potential molecular explanation for its proposed tumor suppressor function.

Fri Mar 29 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: FMRP; fragile X mental retardation protein 1; Fragile X messenger ribonucleoprotein; Fragile X messenger ribonucleoprotein 1; MGC87458; Protein FMR-1

Gene Aliases: FMR1; FMRP; FRAXA; POF; POF1

UniProt ID: (Human) Q06787

Entrez Gene ID: (Human) 2332

Function(s)

G-quadruplex RNA binding chromatin binding RNA binding mRNA binding mRNA 3'-UTR binding protein binding microtubule binding poly(U) RNA binding translation repressor activity translation initiation factor binding RNA strand annealing activity poly(G) binding methylated histone binding siRNA binding miRNA binding RNA stem-loop binding identical protein binding protein homodimerization activity ribosome binding ion channel binding poly(A) RNA binding dynein binding protein heterodimerization activity mRNA 5'-UTR binding sequence-specific mRNA binding translation factor

Process(es)

regulation of alternative mRNA splicing, via spliceosome positive regulation of receptor internalization mRNA processing cellular response to DNA damage stimulus glutamate receptor signaling pathway RNA splicing negative regulation of translation gene silencing by RNA positive regulation of histone phosphorylation cellular response to UV regulation of mRNA stability modulation by host of viral RNA genome replication positive regulation of translation negative regulation of translational initiation regulation of neurotransmitter secretion mRNA transport regulation of filopodium assembly positive regulation of filopodium assembly regulation of gene silencing by miRNA regulation of dendritic spine development positive regulation of dendritic spine development cellular response to hydroxyurea cellular response to virus regulation of neuronal action potential negative regulation of long term synaptic depression positive regulation of intracellular transport of viral material negative regulation of voltage-gated calcium channel activity positive regulation of proteasomal protein catabolic process positive regulation of mRNA binding negative regulation of synaptic vesicle exocytosis positive regulation of gene silencing by miRNA negative regulation of cytoplasmic translation positive regulation of response to DNA damage stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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