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            • Primary Antibodies ›
            • c-Fos Antibodies

            Proteintech

            FOS Monoclonal Antibody (1G2C5)

            53 References
            View all (62) c-Fos antibodies
            Datasheet
            Protocols
            Questions & Answers
            Tech Support
            Catalog # 66590-1-IG
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            150 µL
             
            Datasheet
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            Cite FOS Monoclonal Antibody (1G2C5)

            • Antibody Testing Data (6)
            Application
            FOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Group 53 Created with Sketch.

            FIGURE: 1 / 6

            FOS Antibody (66590-1-IG) in IHC (P)

            Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66590-1-Ig (FOS antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
            View Product

            FOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            FOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            FOS Antibody in Western Blot (WB)
            FOS Antibody in Western Blot (WB)
            FOS Antibody in Western Blot (WB)
            FOS Antibody in Western Blot (WB)

            Product Details

            66590-1-IG

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            View 42 publications 42 publications

            Immunohistochemistry (IHC)

            -
            View 5 publications 5 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:250-1:1,000
            -

            Immunocytochemistry (ICC/IF)

            -
            View 6 publications 6 publications
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            1G2C5

            Immunogen

            FOS Fusion Protein Ag24340 (196-341 aa encoded by BC004490)
            View immunogen

            Conjugate

            Unconjugated
            • CoraLite Plus 488
            • Request custom conjugation

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Protein A

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            Immunogen sequence: ILAAHRPAC KIPDDLGFPE EMSVASLDLT GGLPEVATPE SEEAFTLPLL NDPEPKPSVE PVKSISSMEL KTEPFDDFLF PASSRPSGSE TARSVPDMDL SGSFYAADWE PLHSGSLGMG PMATELEPLC TPVVTCTPSC TAYTSSF (196-341 aa encoded by BC004490 )

            Target Information

            FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: activator protein 1; c-fos oncogene; cellular oncogene c-fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FBJ osteosarcoma oncogene; Fos proto-oncogene, AP-1 trancription factor subunit; Fos proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 7; Protein c-Fos; Proto-oncogene c-Fos; proto-oncogene cFOS; proto-oncogene protein c-fos; Transcription factor AP-1 subunit c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog

            View more View less

            Gene Aliases: AP-1; C-FOS; cFos; D12Rfj1; FOS; G0S7; p55

            View more View less

            UniProt ID: (Human) P01100, (Mouse) P01101, (Rat) P12841

            View more View less

            Entrez Gene ID: (Human) 2353, (Mouse) 14281, (Rat) 314322

            View more View less

            Function(s)

            RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding transcription regulatory region DNA binding protein heterodimerization activity R-SMAD binding DNA binding double-stranded DNA binding sequence-specific DNA binding basic leucine zipper transcription factor

            Process(es)

            conditioned taste aversion DNA methylation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response transforming growth factor beta receptor signaling pathway nervous system development female pregnancy aging response to cold response to light stimulus response to gravity response to toxic substance sleep cellular response to extracellular stimulus response to lipopolysaccharide response to progesterone cellular response to hormone stimulus response to cytokine cellular response to reactive oxygen species response to immobilization stress skeletal muscle cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway response to drug positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of sequence-specific DNA binding transcription factor activity response to corticosterone response to cAMP SMAD protein signal transduction cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of transcription, DNA-templated response to mechanical stimulus response to organic cyclic compound
            It has to be done as per old AB suggested Products section.
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