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Invitrogen

FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02)

Name: FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02)
Brand: Invitrogen
SKU: MA532556
Price: 496.0 USD

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View all (51) FOXA1 antibodies

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Catalog # MA5-32556

Price (USD)

496.00

100 µL

Estimated availability date: 17-Jun-2025

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)
Advanced Verification (2)

Application

FOXA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

FOXA1 Antibody (MA5-32556) in ICC/IF

Immunofluorescence analysis of FOXA1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02) (Product # MA5-32556) at 1:50-1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More

FOXA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FOXA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across MCF7, T-47D, LNCaP, SW480, HCT-116 and SHSY-5Y owing to their inherent genetic constitution. Relative expression of Ci-fkh; fkd7; fkh; fork head domain; forkhead box A1; forkhead box protein A1; forkhead-7; Foxa1; FoxA4a; hepatocyte nuclear factor 3 alpha (winged helix transcription factor); hepatocyte nuclear factor 3, alpha; hepatocyte nuclear factor 3-alpha; HNF3A; HNF-3A; HNF3alpha; HNF-3-alpha; HNF3-alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF3A; Tcf-3a; tFoxA1; Transcription factor 3A; xfkh1; xfkh2 was observed in MCF7, T-47D, LNCaP and SW480 using Anti-FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02) (Product # MA5-32556) in Western Blot. {RE}

Product Details

MA5-32556

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JF10-02

Immunogen

Recombinant protein within Human FOXA1 aa 320-472

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809833

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ci-fkh; fkd7; fkh; fork head domain; Forkhead box protein A1; forkhead-7; FoxA4a; hepatocyte nuclear factor 3 alpha (winged helix transcription factor); hepatocyte nuclear factor 3, alpha; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; HNF3-alpha; HNF3alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF-3A; tFoxA1; Transcription factor 3A; xfkh1; xfkh2

Gene Aliases: FOXA1; Hnf-3a; HNF3A; Tcf-3a; TCF3A

UniProt ID: (Human) P55317, (Mouse) P35582, (Rat) P23512

Entrez Gene ID: (Human) 3169, (Mouse) 15375, (Rat) 25098

Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding sequence-specific DNA binding transcription regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding double-stranded DNA binding winged helix/forkhead transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter chromatin remodeling transcription from RNA polymerase II promoter Notch signaling pathway negative regulation of epithelial to mesenchymal transition covalent chromatin modification dorsal/ventral neural tube patterning response to estradiol positive regulation of intracellular estrogen receptor signaling pathway hormone metabolic process glucose homeostasis positive regulation of neuron differentiation positive regulation of smoothened signaling pathway positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter anatomical structure formation involved in morphogenesis neuron fate specification positive regulation of sequence-specific DNA binding transcription factor activity epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development epithelial-mesenchymal signaling involved in prostate gland development prostate gland epithelium morphogenesis prostate gland stromal morphogenesis epithelial cell maturation involved in prostate gland development alveolar secondary septum development dopaminergic neuron differentiation respiratory basal cell differentiation positive regulation of cell-cell adhesion mediated by cadherin transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development regulation of gene expression chromatin modification lung development tube morphogenesis regulation of cell cycle connective tissue development

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