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Invitrogen

FOXA2 Polyclonal Antibody

1 Published Figure

1 Reference

View all (32) FOXA2 antibodies

Datasheet

Protocols

Questions & Answers

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Catalog # PA5-35033

Price (EUR)

566,00

400 µL

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Published Figures (1)

Application

FOXA2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

FOXA2 Antibody (PA5-35033) in ICC/IF

Immunocytochemistry analysis of FOXA2 in A549 cells. Samples were incubated with FOXA2 polyclonal antibody (Product # PA5-35033) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). FOXA2... View More

FOXA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-35033

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

1:10-1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 128-157 amino acids from the central region of human FOXA2

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2552343

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin. They also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.

Scientific reports

Kimura K,Koizumi T,Urasawa T,Ohta Y,Takakura D,Kawasaki N

PA5-35033 was used in Western Blot to conduct sequential LC/MS/MS analyses of tryptic digest, enriched glycopeptides, and deglycosylated peptides of proteins derived from human-induced pluripotent stem cells (iPSCs) and iPSC-derived neuronal cells.

Thu May 27 00:00:00 UTC 2021

Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.

Scientific reports

Kimura K,Koizumi T,Urasawa T,Ohta Y,Takakura D,Kawasaki N

Thu May 27 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: FKHL20; Forkhead box protein A2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; MGC19807; RONU; TCF-3B; Transcription factor 3B; WHN

Gene Aliases: FOXA2; HNF3B; TCF3B

UniProt ID: (Human) Q9Y261

Entrez Gene ID: (Human) 3170

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding transcription regulatory region DNA binding winged helix/forkhead transcription factor

Process(es)

positive regulation of transcription from RNA polymerase II promoter by glucose negative regulation of transcription from RNA polymerase II promoter by glucose cell fate specification transcription, DNA-templated regulation of transcription from RNA polymerase II promoter adult locomotory behavior negative regulation of epithelial to mesenchymal transition covalent chromatin modification regulation of blood coagulation endocrine pancreas development negative regulation of glucokinase activity positive regulation of embryonic development negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of insulin secretion involved in cellular response to glucose stimulus response to interleukin-6 dopaminergic neuron differentiation primitive streak formation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of gastrulation negative regulation of detection of glucose

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