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Product Details
MA1-23232
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4E7
Immunogen
Amino acids 242-655 of FKHR expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_558989
Product Specific Information
Predicted molecular weight: ~70 kDa. Positive Control: Cos 1 cells transfected with human FKHR.
Target Information
FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOX01A
Gene Aliases: FKH1; FKHR; FOXO1; FOXO1A
UniProt ID: (Human) Q12778
Entrez Gene ID: (Human) 2308
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, transcription factor binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator binding
chromatin binding
protein binding
beta-catenin binding
ubiquitin protein ligase binding
sequence-specific DNA binding
protein phosphatase 2A binding
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
temperature homeostasis
cellular glucose homeostasis
transcription, DNA-templated
protein acetylation
autophagy
apoptotic process
cellular response to DNA damage stimulus
insulin receptor signaling pathway
cellular response to starvation
positive regulation of autophagy
endocrine pancreas development
cellular response to insulin stimulus
negative regulation of stress-activated MAPK cascade
cellular response to oxidative stress
regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter
positive regulation of apoptotic process
negative regulation of apoptotic process
fat cell differentiation
negative regulation of fat cell differentiation
positive regulation of gluconeogenesis
positive regulation of protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
enamel mineralization
cellular response to hydrogen peroxide
cellular response to cold
cellular response to hyperoxia
cellular response to dexamethasone stimulus
cellular response to nitric oxide
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
response to fluoride
regulation of neural precursor cell proliferation
regulation of reactive oxygen species metabolic process
regulation of energy homeostasis
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