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Invitrogen

FRS2 Polyclonal Antibody

1 Reference

View all (24) FRS2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

FRS2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 1

FRS2 Antibody (PA1-24685) in ICC/IF

Immunofluorescence analysis FRS2 Polyclonal Antibody was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled FRS2 polyclonal antibody (Product PA1-24685) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175) at... View More

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Product Details

PA1-24685

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

10-20 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to human FRS2 sequence, (amino acids 487-505).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.9 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2247174

Product Specific Information

PA1-24685 detects a band of ~92 and 95 kDa. Positive control of rat PC-12 and human HepG2 cell lysates suggested.

Target Information

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Kinetics of receptor tyrosine kinase activation define ERK signaling dynamics.

Science signaling

Kiyatkin A,van Alderwerelt van Rosenburgh IK,Klein DE,Lemmon MA

PA1-24685 was used in Western Blot to investigate if receptor tyrosine kinases activation kinetics play a crucial role in determining MAP kinase cascade signaling dynamics and cell fate decisions, and if signaling outcome can be modified by activating a given receptor tyrosine kinases in different ways.

Tue Aug 18 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: FGFR signalling adaptor; FGFR substrate 2; FGFR-signaling adaptor SNT; Fibroblast growth factor receptor substrate 2; FRS2-alpha; SNT-1; SNT2; Suc1-associated neurotrophic factor target 1

Gene Aliases: 4732458E18; C330018A15Rik; FRS1A; FRS2; FRS2A; FRS2alpha; SNT; SNT-1; SNT1

UniProt ID: (Human) Q8WU20, (Mouse) Q8C180

Entrez Gene ID: (Human) 10818, (Mouse) 327826, (Rat) 314850

Function(s)

transmembrane receptor protein tyrosine kinase adaptor activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding insulin receptor binding neurotrophin TRKA receptor binding protein binding 1-phosphatidylinositol-3-kinase activity phosphatase activator activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

activation of MAPK activity gastrulation with mouth forming second organ induction lens development in camera-type eye ventricular septum development transmembrane receptor protein tyrosine kinase signaling pathway neuroblast proliferation fibroblast growth factor receptor signaling pathway anterior/posterior axis specification, embryo positive regulation of signal transduction forebrain development regulation of apoptotic process optic placode formation involved in camera-type eye formation regulation of epithelial cell proliferation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis lens fiber cell development regulation of ERK1 and ERK2 cascade MAPK cascade activation of MAPKK activity transmembrane receptor protein tyrosine phosphatase signaling pathway G-protein coupled receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol-3-phosphate biosynthetic process positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling

Performance Guarantee

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