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Application
FIGURE: 1 / 1
FSTL3 Antibody (PA5-79285) in WB
Cancer medicine 2023 -
8 FIGURE Expression verification of stemness-related prognostic genes. (A) The spheroid formation assay of HCT116 and DLD1. (B) The expressions of TIMP1, PGF, FSTL3, SNAI1, and FOXC1 in NCM460, HCT116, and DLD1 detected by qRT-PCR. (C) The expressions of TIMP1, PGF, FSTL3, SNAI1, and FOXC1 in NCM460, HCT116, and DLD1 detected by western blot; compared with NCM460, *** p < 0.001; compared with DLD1, ### p < ... View More
Product Details
PA5-79285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FSTL3 (145-159aa ECELRAARCRGHPDL).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746401
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U2OS whole cell, human Hela whole cell, human A549 whole cell, human PC-3 whole cell, human HepG2 whole cell, human K562 whole cell, rat liver tissue, rat testis tissue, rat NRK whole cell, mouse liver tissue, mouse testis tissue, mouse lung tissue.
Target Information
Follistatin-like 3 (FSTL3) is a gene located on chromosome 19p13.3 and encodes a secreted glycoprotein that belongs to the follistatin-related protein family. FSTL3 functions as an antagonist to members of the transforming growth factor-beta (TGF-β) superfamily, particularly inhibiting activin and myostatin signaling. This inhibitory action is crucial for regulating muscle growth, fertility, and inflammation. FSTL3 is expressed in various tissues, including the placenta, gonads, and skeletal muscle, where it modulates physiological processes by binding to and neutralizing the activities of its target ligands. Dysregulation of FSTL3 has been implicated in conditions such as muscle wasting, metabolic syndrome, and reproductive disorders, reflecting its role in maintaining tissue homeostasis and function. Research into FSTL3 focuses on its potential therapeutic applications in diseases involving TGF-β pathway dysregulation, offering insights into strategies for modulating growth factor activities in clinical settings.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like protein 3; Follistatin-related gene protein; Follistatin-related protein 3
Gene Aliases: E030038F23Rik; FLRG; FSRP; FSTL3; UNQ674/PRO1308
UniProt ID: (Human) O95633, (Mouse) Q9EQC7, (Rat) Q99PW7
Entrez Gene ID: (Human) 10272, (Mouse) 83554, (Rat) 114031
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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