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Invitrogen
FUS Monoclonal Antibody (1FU-1D2)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
FUS Antibody (MA3-089) in ICC/IF
Immunofluorescence of FUS was performed using HeLa wild-type and FUS KO cells that were transfected with a green or a far red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the FUS monoclonal antibody (Product # MA3-... View More
Product Details
MA3-089
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1FU-1D2
Immunogen
Synthetic peptide (GPRDQGSRHDSEQDNSDC) coupled to ovalbumin. From aa 266 to aa 283 of FUS
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2633334
Product Specific Information
MA3-089 detects AID from human samples
MA3-089 has been successfully used in Western blot.
The MA3-089 immunogen is the synthetic peptide coupled to ovalalbumin, aa 266 to 283. MA3-089 detects FUS/TLS (~70kDa) in human samples.
Target Information
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 75 kDa DNA-pairing protein; fus-like protein; fused in sarcoma; fusion gene in myxoid liposarcoma; heterogeneous nuclear ribonucleoprotein P2; Oncogene FUS; Oncogene TLS; POMp75; RNA-binding protein FUS; Translocated in liposarcoma protein
Gene Aliases: ALS6; ETM4; FUS; FUS1; HNRNPP2; POMP75; TLS
UniProt ID: (Human) P35637
Entrez Gene ID: (Human) 2521
nucleotide binding
DNA binding
transcription coactivator activity
RNA binding
protein binding
zinc ion binding
estrogen receptor binding
myosin V binding
ionotropic glutamate receptor binding
identical protein binding
poly(A) RNA binding
retinoid X receptor binding
thyroid hormone receptor binding
RNA metabolism protein
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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