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Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
The antiSerum does not cross-react with any other Human plasma proteins as tested in geldiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a weak reaction with Mouse has been observed.
In immunoelectrophoresis use 2 µL human serum against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in a 3 mm diameter center well and 10 µL of the antiserum in 3 mm diameter peripheral wells. In single radial immunodiffusion (Mancini) use 1% antiserum in the gel. Preciptin titre not less than 1:8 when tested against normal human serum in agar block immunodiffusion titration.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Factor B is a component of the alternative complement pathway. The protein is a single chain polypeptide with a molecular mass of 93 kDa. Factor B is cleaved by factor D into the Ba component (30 kDa) and the Bb (63 kDa). Bb together with C3b forms the alternative pathway C3 convertase C3bBb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-factor, properdin; C3 proaccelerator; C3 proactivator; C3/C5 convertase; Complement factor B; DADB-122G4.5; GBG; Glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein; PBF2; Properdin factor B
Gene Aliases: AHUS4; ARMD14; BF; BFD; CFAB; CFB; CFBD; FB; FBI12; GBG; H2-Bf; PBF2
UniProt ID: (Human) P00751
Entrez Gene ID: (Human) 629
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