Loading ...

Invitrogen

Factor IX Monoclonal Antibody (5041)

Name: Factor IX Monoclonal Antibody (5041)
Brand: Invitrogen
SKU: MA143012
Price: 441.0 USD

1 Published Figure

5 References

View all (24) Factor IX antibodies

Datasheet

Protocols

Questions & Answers

Catalog # MA1-43012

Price (USD)

441.00

100 µg

Estimated availability date: 23-Jun-2025

Check your price

Request bulk or custom format

Join Aspire^™ for free antibodies

Datasheet

Protocols

Questions & Answers

Published Figures (1)

Application

FIGURE: 1 / 1

Factor IX Antibody (MA1-43012) in ELISA

Nature communications 2020 - Fig. 3 F9 KI into the alpha-globin locus results in expression and secretion of functional enzyme. a AAV6 donor used for KI experiments of FIX Padua. b FIX KI efficiency in HUDEP-2 cells was measured by flow cytometry (light green) or ddPCR specific for on-target integration (dark green) before and after sorting ( n = 1). c Quantification of FIX mRNA in KI HUDEP-2 upon differentiation (mean +- SD, n = 2 und... View More

PMID: 32728076

View Product

Product Image Gallery

Product Details

MA1-43012

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

5-10 µg/mL

View 4 publications 4 publications

Radioimmune Assays (RIA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5041

Immunogen

Heavy chain of human factor IX and Ixa.

Conjugate

Unconjugated

Form

Liquid

Concentration

9.5 mg/mL

Purification

SDS-PAGE

Storage buffer

50% water with 50% glycerol

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1074312

Product Specific Information

MA1-43012 detects Factor IX in human samples, human factor Ixa and the heavy chain of human factor IX and IXa.

MA1-43012 has successfully been used in radioimmune assays, ELISA, Western blot and immunohistochemistry applications.

MA1-43012 was derived from human factor IX.

Target Information

Coagulation factor IX is a single chain polypeptide with a molecular mass of 57 kDa. Normal concentrations of factor IX in human plasma is approximately 3 µg/mL. Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins.

Nature communications

Pavani G,Laurent M,Fabiano A,Cantelli E,Sakkal A,Corre G,Lenting PJ,Concordet JP,Toueille M,Miccio A,Amendola M

MA1-43012 was used in Western Blotting to describe an ex vivo editing approach to achieve efficient gene targeting in human hematopoietic stem/progenitor cells (HSPCs) and robust expression of clinically relevant proteins by the erythroid lineage.

Wed Jul 29 00:00:00 UTC 2020

Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins.

Nature communications

Pavani G,Laurent M,Fabiano A,Cantelli E,Sakkal A,Corre G,Lenting PJ,Concordet JP,Toueille M,Miccio A,Amendola M

Wed Jul 29 00:00:00 UTC 2020

Preclinical Development of an AAV8-hUGT1A1 Vector for the Treatment of Crigler-Najjar Syndrome.

Molecular therapy. Methods & clinical development

Collaud F,Bortolussi G,Guianvarc'h L,Aronson SJ,Bordet T,Veron P,Charles S,Vidal P,Sola MS,Rundwasser S,Dufour DG,Lacoste F,Luc C,Wittenberghe LV,Martin S,Le Bec C,Bosma PJ,Muro AF,Ronzitti G,Hebben M,Mingozzi F

MA1-43012 was used in an ELISA assay to establish long-term safety and efficiency of gene transder with single strand AAV8-hUGT1A1 in animal models of hemophilia disease.

Fri Mar 15 00:00:00 UTC 2019

Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration.

Nature communications

Meliani A,Boisgerault F,Hardet R,Marmier S,Collaud F,Ronzitti G,Leborgne C,Costa Verdera H,Simon Sola M,Charles S,Vignaud A,van Wittenberghe L,Manni G,Christophe O,Fallarino F,Roy C,Michaud A,Ilyinskii P,Kishimoto TK,Mingozzi F

MA1-43012 was used in Enzyme-linked immunosorbent assay to demonstrate that synthetic vaccine particles encapsulating rapamycin, co-administered with adeno-associated virus vectors, prevents the induction of anti-capsid humoral and cell-mediated responses.

Fri Oct 05 00:00:00 UTC 2018

Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration.

Nature communications

Fri Oct 05 00:00:00 UTC 2018

Influence of Pre-existing Anti-capsid Neutralizing and Binding Antibodies on AAV Vector Transduction.

Molecular therapy. Methods & clinical development

Fitzpatrick Z,Leborgne C,Barbon E,Masat E,Ronzitti G,van Wittenberghe L,Vignaud A,Collaud F,Charles S,Simon Sola M,Jouen F,Boyer O,Mingozzi F

MA1-43012 was used in an ELISA assay to highlight a virtually opposite effect of neutralizing and binding antibodies on AAV vectors transduction.

Fri Jun 15 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Christmas factor; Coagulation factor IX; EC 3.4.21.22; factor 9; factor IX F9; Factor9; FIX F9; plasma thromboplastic component; Plasma thromboplastin component; PTC; RP6-88D7.1; serine protease

Gene Aliases: F9; F9 p22; FIX; HEMB; P19; PTC; THPH8

UniProt ID: (Human) P00740

Entrez Gene ID: (Human) 2158

Function(s)

Process(es)

signal peptide processing proteolysis ER to Golgi vesicle-mediated transport blood coagulation blood coagulation, intrinsic pathway blood coagulation, extrinsic pathway peptidyl-glutamic acid carboxylation zymogen activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Factor IX Monoclonal Antibody (5041)

Factor IX Antibody (MA1-43012) in ELISA

Product Details

MA1-43012

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Radioimmune Assays (RIA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!