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Invitrogen
FceR1 alpha Monoclonal Antibody (MAR-1), Super Bright™ 436, eBioscience™
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Application
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FceR1 alpha Antibody (62-5898-82) in Flow
MC/9 cells (a mouse mast cell line) were stained with Armenian Hamster IgG Isotype Control, Super Bright 436 (Product # 62-4888-82) (blue histogram) or Fc epsilon Receptor I alpha (FceR1) Monoclonal Antibody, Super Bright 436 (purple histogram). Total viable cells were used for analysis.
Product Details
62-5898-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MAR-1
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762755
Product Specific Information
Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported: The MAR-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: fc epsilon r1; Fc-epsilon RI-alpha; FcERI; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; RP11-550P17.3
Gene Aliases: Fce1a; fcepsilonri; Fcer1a; FcERI; Fcr-5
UniProt ID: (Mouse) P20489
Entrez Gene ID: (Mouse) 14125
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