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Invitrogen
Filaggrin Polyclonal Antibody
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Application
FIGURE: 1 / 2
Filaggrin Antibody (PA5-116911) in WB
Western blot analysis of Filaggrin in various lysates. Samples were incubated with Filaggrin Polyclonal antibody (Product # PA5-116911) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-116911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human FLG (NP_0020071)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.89 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901541
Product Specific Information
Positive Samples: A375, A431, HepG2, TE-1, SGC7901, mouse stomach, mouse liver, rat liver
Immunogen sequence: MSTLLENIFA IINLFKQYSK KDKNTDTLSK KELKELLEKE FRQILKNPDD PDMVDVFMDH LDIDHNKKID FTEFLLMVFK LAQAYYESTR KE
Target Information
Filaggrin is expressed only in well differentiated keratinized epithelial cells. The synthesis of filaggrin varies in types of ichthyosis, and with viral, premalignant and malignant conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epidermal filaggrin; Filaggrin; Filaggrin (profilaggrin)
Gene Aliases: ATOD2; AW107830; FLG; ft; Pflg
UniProt ID: (Human) P20930
Entrez Gene ID: (Human) 2312, (Rat) 24641, (Mouse) 14246
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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