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Clone 33B12 and 117C9 detect overlapping epitopes on the Forssman glycolipid hapten (GalNA1- c3aG alNAcD1 -3GalaI-4GalB1-4GlcBl-lCer). Clone 117C9 recognizes the internal sugar sequence GalNAqYlBGal. The 117C9 antibody does not react exclusively with the Forssman antigen. In.a lipid extract fractionated by Folch partition of Mouse mammary tumors the antibody also detects other glycolipid.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Has lost the ability to synthesize Forssman glycolipid antigen (FORS1/FG. Might have acquired an alternative function in glycosphingolipid metabolism, but it remains to be established. It appears to have drifted more slowly than confirmed pseudogenes in the glycosyltransferase 6 family, suggesting that it has remained under evolutionary pressure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Forssman glycolipid synthase; forssman glycolipid synthetase; Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
Gene Aliases: Fgs; Fs; Gbgt1
UniProt ID: (Mouse) Q8VI38
Entrez Gene ID: (Mouse) 227671
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