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Invitrogen

G6PD Polyclonal Antibody

Name: G6PD Polyclonal Antibody
Brand: Invitrogen
SKU: PA527359
Price: 548.0 USD

Advanced Verification

3 Published Figures

4 References

View all (37) G6PD antibodies

Datasheet

Protocols

Questions & Answers

Catalog # PA5-27359

Price (USD)

548.00

100 µL

Estimated availability date: 12-Jun-2025

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Datasheet

Protocols

Questions & Answers

Application

G6PD Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

G6PD Antibody (PA5-27359) in ICC/IF

Immunofluorescence analysis of G6PD was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with G6PD Goat Polyclonal Antibody (Product # PA5-27359) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a diluti... View More

G6PD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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G6PD Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of G6PD was observed in Mouse Adipose in comparison to Mouse Heart using Anti-G6PD Polyclonal Antibody (Product # PA5-27359) in Western Blot. G6PD expression was induced when 3T3-L1 fibroblasts were differentiated to Adipocytes, which also demonstrates antibody specificity by relative expression. {RE}

Product Details

PA5-27359

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.81 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544835

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Rat (93%), Xenopus laevis (82%), Rabbit (95%), Sheep (92%), Rhesus Monkey (99%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Functional effects of an African glucose-6-phosphate dehydrogenase (G6PD) polymorphism (Val68Met) on red blood cell hemolytic propensity and post-transfusion recovery.

Transfusion

Wang L,Rochon ER,Gingras S,Zuchelkowski BE,Sinchar DJ,Alipour E,Reisz JA,Yang M,Page GP,Kanias T,Triulzi DJ,Lee JS,Kim-Shapiro DB,D'Alessandro A,Gladwin MT

Published figure using G6PD polyclonal antibody (Product # PA5-27359) in Western Blot

Mon Apr 01 00:00:00 UTC 2024

Proteomic profiling of small extracellular vesicles derived from mouse pancreatic cancer and stellate cells: Role in pancreatic cancer.

Proteomics

Perera CJ,Hosen SZ,Khan T,Fang H,Mekapogu AR,Xu Z,Falasca M,Chari ST,Wilson JS,Pirola R,Greening DW,Apte MV

Published figure using G6PD polyclonal antibody (Product # PA5-27359) in Western Blot

Sat Jun 01 00:00:00 UTC 2024

p63 isoforms regulate metabolism of cancer stem cells.

Journal of proteome research

D'Aguanno S,Barcaroli D,Rossi C,Zucchelli M,Ciavardelli D,Cortese C,De Cola A,Volpe S,D'Agostino D,Todaro M,Stassi G,Di Ilio C,Urbani A,De Laurenzi V

PA5-27359 was used in western blot to study the modulation of colon cancer stem cell metabolism by p63

Fri Apr 04 00:00:00 UTC 2014

Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.

Cell death & disease

Ciavardelli D,Rossi C,Barcaroli D,Volpe S,Consalvo A,Zucchelli M,De Cola A,Scavo E,Carollo R,D'Agostino D,Forlì F,D'Aguanno S,Todaro M,Stassi G,Di Ilio C,De Laurenzi V,Urbani A

PA5-27359 was used in western blot to identify the metabolic phenotype associated with breast cancer stem cells

Thu Jul 17 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: G6PD; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase X; glucose-6-phosphate dehydrogenase X-linked

Gene Aliases: G28A; G6PD; G6pd-1; G6PD1; G6pdx; Gpdx

UniProt ID: (Human) P11413, (Rat) P05370, (Mouse) Q00612

Entrez Gene ID: (Human) 2539, (Rat) 24377, (Mouse) 14381

Function(s)

glucose-6-phosphate dehydrogenase activity protein binding glucose binding identical protein binding protein homodimerization activity NADP binding carbohydrate binding oxidoreductase activity dehydrogenase

Process(es)

glucose metabolic process pentose-phosphate shunt lipid metabolic process cholesterol biosynthetic process NADP metabolic process NADPH regeneration glutathione metabolic process pentose-phosphate shunt, oxidative branch negative regulation of protein glutathionylation response to organic cyclic compound pentose biosynthetic process substantia nigra development response to food cellular response to oxidative stress erythrocyte maturation regulation of neuron apoptotic process response to ethanol ribose phosphate biosynthetic process glucose 6-phosphate metabolic process oxidation-reduction process cytokine production angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure vasodilation by angiotensin involved in regulation of systemic arterial blood pressure NADP biosynthetic process response to oxidative stress interleukin-10 production interleukin-12 production regulation of multicellular organism growth erythrocyte development carbohydrate metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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