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            • Primary Antibodies ›
            • GAPDH Antibodies

            Proteintech

            GAPDH Monoclonal Antibody (1E6D9), HRP

            495 References
            View all (122) GAPDH antibodies

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            Datasheet
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            Catalog # HRP-60004
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            Price (USD)
            524.00
            100 µL
             
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            Cite GAPDH Monoclonal Antibody (1E6D9), HRP

            • Antibody Testing Data (2)
            Application
            GAPDH Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            GAPDH Antibody (HRP-60004) in WB

            HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
            View Product

            GAPDH Antibody in Western Blot (WB)
            GAPDH Antibody in Western Blot (WB)

            Product Details

            HRP-60004

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:5,000-1:50,000
            View 470 publications 470 publications

            Immunohistochemistry (IHC)

            -
            View 3 publications 3 publications

            Immunocytochemistry (ICC/IF)

            -
            View 1 publication 1 publication

            Immunoprecipitation (IP)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 4 publications 4 publications

            Not applicable (N/A)

            -
            View 16 publications 16 publications
            Product Specifications

            Species Reactivity

            Human, Mouse, Plant, Rat, Zebrafish

            Published species

            Hamster, Human, Mouse, Rat

            Host/Isotype

            Mouse / IgG2b

            Class

            Monoclonal

            Type

            Antibody

            Clone

            1E6D9

            Immunogen

            Recombinant protein

            Conjugate

            HRP
            • Unconjugated
            • CoraLite 594
            • CoraLite Plus 488
            • CoraLite Plus 647
            • CoraLite Plus 670
            • Request custom conjugation

            Form

            Liquid

            Concentration

            0.67 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol, 0.5% BSA

            Contains

            0.02% thimerosal, 0.05% ProClin 300

            Storage conditions

            -20°C, store in dark

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

            Target Information

            GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

            ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

            View more View less

            Gene Aliases: BARS-38; bb02e05; cb350; cb609; CDABP0047; fb71f08; fk58c09; G3PD; g3pdh; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; mg:bb02e05; OK/SW-cl.12; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908

            View more View less

            UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467, (Zebrafish) Q5XJ10, (Zebrafish) Q5MJ86

            View more View less

            Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447, (Zebrafish) 317743, (Zebrafish) 406367

            View more View less

            Function(s)

            glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor transferase activity nucleotide binding dehydrogenase

            Process(es)

            microtubule cytoskeleton organization gluconeogenesis regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process canonical glycolysis cellular response to interferon-gamma spermatid development sperm motility positive regulation of glycolytic process carbohydrate metabolic process glycolytic process apoptotic process multicellular organism development oxidation-reduction process response to ammonium ion regulation of translation glucose metabolic process
            It has to be done as per old AB suggested Products section.
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