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Application
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GAS2L1 Antibody (PA5-96067) in WB
Western blot analysis of GAS2L1 in extracts of various cell lines. Samples were incubated with GAS2L1 Polyclonal antibody (Product # PA5-96067) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-96067
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 130-400 of human GAS2L1 (NP_0064692)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.71 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807869
Product Specific Information
Immunogen sequence: RKNEKSVVLC LLEVARRGAR LGLLAPRLVQ FEQEIERELR AAPPAPNAPA AGEDTTETAP APGTPARGPR MTPSDLRNLD ELVREILGRC TCPDQFPMIK VSEGKYRVGD SSLLIFVRVL RSHVMVRVGG GWDTLEHYLD KHDPCRCSST AHRPPQPRVC TFSPQRVSPT TSPRPASPVP GSERRGSRPE MTPVSLRSTK EGPETPPRPR DQLPPHPRSR RYSGDSDSSA SSAQSGPLGT RSDDTGTGPR RERPSRRLTT GTPASPRRPP A; Positive Samples: A673, 22Rv1; Cellular Location: Cytoplasm, cytoskeleton
Target Information
The Gas2 protein is thought to play a role in apoptosis by acting as a cell death substrate for caspases. Gas2, a component of the microfilament system, is cleaved by a caspase (caspase-3 or caspase-7) at Asparagine 278 during apoptosis. The cleaved form of Gas2 dramatically induces the re-arrangement of the Actin cytoskeleton and causes potent changes in the shape of the affected cells. Gas2 is a cytoskeleton and peripheral membrane protein that co-localizes with Actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Gas2 is mainly membrane-associated, but when hyperphosphorylated it will accumulate at membrane ruffles, indicating Gas2 involvement in that process. Gar22 (Gas2-related protein on chromosome 22), also designated growth arrest-specific protein 2-like 1 (Gas2-like1), belongs to the Gas2 family and contains one CH (calponinhomo-logy) domain. It is thought to be involved in the cross-linking of microtubules and microfilaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GAS2-like protein 1; GAS2-related protein on chromosome 22; growth arrest-specific 2 like 1; Growth arrest-specific protein 2-like 1
Gene Aliases: GAR22; GAS2L1
UniProt ID: (Human) Q99501
Entrez Gene ID: (Human) 10634
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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