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Invitrogen

GCLC Polyclonal Antibody

12 References

View all (20) GCLC antibodies

Datasheet

Protocols

Datasheet

Protocols

Antibody Testing Data (2)

Application

GCLC Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

GCLC Antibody (PA5-16581) in ICC/IF

Immunofluorescence analysis of Gamma-Glutamyl cysteine Synthetase/Glutamate-cysteine was performed using70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GCLC Rabbit Polyclonal Antibody (Product # PA5-16581) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary ... View More

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Product Details

PA5-16581

Applications

Tested Dilution

Publications

Western Blot (WB)

2.5-5.0 µg/mL

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

10 µg/mL

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_10977936

Product Specific Information

PA5-16581 targets Gamma-Glutamyl cysteine Synthetase/Glutamate-cysteine Ligase in IP and WB applications and shows reactivity with Bovine, Human, mouse, Non-human primate, and Rat samples.

The PA5-16581 immunogen is a synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS).

Target Information

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis.

Scientific reports

Wasik U,Milkiewicz M,Kempinska-Podhorodecka A,Milkiewicz P

PA516581 was used in western blot to find primary biliary cholangitis is associated with reduced nuclear factor (erythroid-derived 2)-like 2 expression

Thu Mar 23 00:00:00 UTC 2017

Does hepatic oxidative stress enhance activation of nuclear factor-E2-related factor in patients with nonalcoholic steatohepatitis?

Antioxidants & redox signaling

Takahashi Y,Kobayashi Y,Kawata K,Kawamura K,Sumiyoshi S,Noritake H,Watanabe S,Chida T,Souda K,Sakaguchi T,Nakamura H,Suda T

PA5-16581 was used in western blot to study the effects of oxidative stress on the expression of Nrf2 in the livers of patients with non-alcoholic steatohepatitis

Mon Jan 20 00:00:00 UTC 2014

Alterations in the metabolomics of sulfur-containing substances in rat kidney by betaine.

Amino acids

Kim YC,Kwon DY,Kim JH

PA5-16581 was used in western blot to study the effects of betaine administration on rat kidney sulfur compound metabolomics

Tue Apr 01 00:00:00 UTC 2014

Cross-regulations among NRFs and KEAP1 and effects of their silencing on arsenic-induced antioxidant response and cytotoxicity in human keratinocytes.

Environmental health perspectives

Zhao R,Hou Y,Zhang Q,Woods CG,Xue P,Fu J,Yarborough K,Guan D,Andersen ME,Pi J

PA5-16581 was used in western blot to study the regulatory interactions of NRF2, NRF1, and KEAP1 and their role in the anti-oxidant responses of human keratinocytes to arsenic toxicity

Sun Apr 01 00:00:00 UTC 2012

Isolation, identification, and biological evaluation of Nrf2-ARE activator from the leaves of green perilla (Perilla frutescens var. crispa f. viridis).

Free radical biology & medicine

Izumi Y,Matsumura A,Wakita S,Akagi K,Fukuda H,Kume T,Irie K,Takada-Takatori Y,Sugimoto H,Hashimoto T,Akaike A

PA5-16581 was used in western blot to study the ability of a green perilla leaf extract to activate the Nrf2-ARE pathway

Wed Aug 15 00:00:00 UTC 2012

Acute exercise stress activates Nrf2/ARE signaling and promotes antioxidant mechanisms in the myocardium.

Free radical biology & medicine

Muthusamy VR,Kannan S,Sadhaasivam K,Gounder SS,Davidson CJ,Boeheme C,Hoidal JR,Wang L,Rajasekaran NS

PA5-16581 was used in western blot to study the effect of acute exercise stress on Nrf2/ARE signaling and antioxidant mechanisms in the myocardium

Sun Jan 15 00:00:00 UTC 2012

Microtubules as a critical target for arsenic toxicity in lung cells in vitro and in vivo.

International journal of environmental research and public health

Zhao Y,Toselli P,Li W

PA5-16581 was used in western blot to study the role of microtubules as targets of lung cell arsenic toxicity and the mechanisms involved

Wed Feb 01 00:00:00 UTC 2012

Disruption of Nrf2/ARE signaling impairs antioxidant mechanisms and promotes cell degradation pathways in aged skeletal muscle.

Biochimica et biophysica acta

Miller CJ,Gounder SS,Kannan S,Goutam K,Muthusamy VR,Firpo MA,Symons JD,Paine R,Hoidal JR,Rajasekaran NS

PA5-16581 was used in immunohistochemistry and western blot to study the potential role of Nrf2 signaling in age-related ROS accumulation and skeletal muscle myopathy

Fri Jun 01 00:00:00 UTC 2012

Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training.

PloS one

Gounder SS,Kannan S,Devadoss D,Miller CJ,Whitehead KJ,Odelberg SJ,Firpo MA,Paine R,Hoidal JR,Abel ED,Rajasekaran NS

PA5-16581 was used in western blot to study the ability of moderate exercise to reverse the age-related decline in cardiac Nrf2 transcriptional activity and its associated antioxidants

Thu Mar 28 00:00:00 UTC 2013

Increased glutathione synthesis following Nrf2 activation by vanadyl sulfate in human chang liver cells.

International journal of molecular sciences

Kim AD,Zhang R,Kang KA,You HJ,Hyun JW

PA5-16581 was used in western blot to study the effect of vanadyl sulfate on nrf2 activation and glutathione synthesis in human chang liver cells

Thu Jan 22 00:00:00 UTC 2015

Carbon monoxide produced by heme oxygenase-1 in response to nitrosative stress induces expression of glutamate-cysteine ligase in PC12 cells via activation of phosphatidylinositol 3-kinase and Nrf2 signaling.

The Journal of biological chemistry

Li MH,Jang JH,Na HK,Cha YN,Surh YJ

PA5-16581 was used in western blot to study the potential role of carbon monoxide produced by heme oxygenase-1 in the adaptive survival response to peroxynitrite-induced PC12 cell death

Fri Sep 28 00:00:00 UTC 2007

Expression of antioxidant enzymes in rat retinal ischemia followed by reperfusion.

Metabolism: clinical and experimental

Agardh CD,Gustavsson C,Hagert P,Nilsson M,Agardh E

PA5-16581 was used in western blot to examine the role of antioxidant enzymes in rat retinal ischemia-reperfusion injury and recovery

Sat Jul 01 00:00:00 UTC 2006

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