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OriGene
GEMIN8 Monoclonal Antibody (UMAB185), UltraMAB™
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Application
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GEMIN8 Antibody (UM800077) in IHC (P)
Immunohistochemical staining of paraffin-embedded human kidney using anti- GEMIN8 clone UMAB185 mouse monoclonal antibody at 1:400 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM800077 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110°C for 3 min using pressure chamber/cooker. The tubule epithelia cells shows strong membraneous and cytoplasmic staining.
Product Details
UM800077
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
UMAB185
Immunogen
Full length human recombinant protein of human GEMIN8 produced in E.coli.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5-1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: family with sequence similarity 51, member A1; gem (nuclear organelle) associated protein 8; Gem-associated protein 8; Gemin-8; Protein FAM51A1
Gene Aliases: FAM51A1; GEMIN8
UniProt ID: (Human) Q9NWZ8
Entrez Gene ID: (Human) 54960
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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