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Chromotek
GFP VHH Recombinant Alpaca Monoclonal Antibody (CTK0201)
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Product Details
GT-250
Product Specifications
Species Reactivity
Tag
Host/Isotype
Alpaca
Expression System
P. pastoris
Class
Recombinant Monoclonal
Type
Antibody
Clone
CTK0201
Immunogen
full-length GFP from Aequorea victoria
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
25mM TAPS, pH 8.5, with 5mM EDTA, 500mM NaCl
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Wet ice
Product Specific Information
Anti-GFP Nanobody for conjugation to dyes, beads, plates and surfaces via NHS-chemistry.
Affinity: Dissociation constant KD of 1 pM. Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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