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Invitrogen

GLI3 Recombinant Rabbit Monoclonal Antibody (JG83-39)

Advanced Verification

View all (13) GLI3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

Application

FIGURE: 1 / 4

GLI3 Antibody (MA5-34779) in WB

Western blot was performed using Anti-GLI3 Recombinant Rabbit Monoclonal Antibody (JG83-39) (Product # MA5-34779) and a 200 kDa band corresponding to GLI3 was observed across the cell lines tested along with a non-specific band (*) at ~ 25 kDa. Whole-cell extracts (40 µg lysate) of SW480 (Lane 1), and HeLa (Lane 2) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB2100... View More

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GLI3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SW480 cells were transfected with GLI3 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-GLI3 Recombinant Rabbit Monoclonal Antibody (JG83-39) (Product # MA5-34779). {KD}

Product Details

MA5-34779

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JG83-39

Immunogen

Recombinant protein within Human Gli3 aa 1261-1580

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2848687

Product Specific Information

Positive Control: PC-3M.

Target Information

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GLI-Kruppel family member GLI3; GLI3 form of 190 kDa; GLI3 full-length protein; GLI3-190; GLI3FL; glioma-associated oncogene family zinc finger 3; oncogene GLI3; Transcriptional activator GLI3; zinc finger protein GLI3

Gene Aliases: ACLS; GCPS; GLI3; GLI3-190; GLI3FL; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV

UniProt ID: (Human) P10071

Entrez Gene ID: (Human) 2737

Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding histone acetyltransferase binding histone deacetylase binding metal ion binding DNA-binding transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter metanephros development branching involved in ureteric bud morphogenesis in utero embryonic development positive regulation of neuroblast proliferation transcription from RNA polymerase II promoter smoothened signaling pathway axon guidance hindgut morphogenesis heart development negative regulation of cell proliferation anterior/posterior pattern specification proximal/distal pattern formation protein processing optic nerve morphogenesis hippocampus development smoothened signaling pathway involved in ventral spinal cord interneuron specification smoothened signaling pathway involved in spinal cord motor neuron cell fate specification forebrain dorsal/ventral pattern formation layer formation in cerebral cortex forebrain radial glial cell differentiation lateral ganglionic eminence cell proliferation melanocyte differentiation lung development positive regulation of chondrocyte differentiation T cell differentiation in thymus limb morphogenesis wound healing positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth embryonic digit morphogenesis negative regulation of apoptotic process nose morphogenesis tongue development response to estrogen negative thymic T cell selection negative regulation of neuron differentiation positive regulation of osteoblast differentiation negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation embryonic digestive tract morphogenesis embryonic digestive tract development developmental growth camera-type eye morphogenesis embryonic skeletal system morphogenesis oligodendrocyte differentiation palate development frontal suture morphogenesis lambdoid suture morphogenesis sagittal suture morphogenesis mammary gland specification smoothened signaling pathway involved in dorsal/ventral neural tube patterning artery development anterior semicircular canal development lateral semicircular canal development cell differentiation involved in kidney development thymocyte apoptotic process negative regulation of canonical Wnt signaling pathway regulation of bone development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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