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Invitrogen
GLS2 Polyclonal Antibody
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Application
FIGURE: 1 / 8
GLS2 Antibody (PA5-115292) in ICC/IF
Immunocytochemistry analysis of GLS2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115292). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115292
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GLS2(Accession Q9UI32), corresponding to amino acid residues L31-A81.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899928
Product Specific Information
Antibody detects endogenous levels of total GLS2.
Target Information
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well. At least one transcribed pseudogene has been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: breast cell glutaminase; GLS; glutaminase 2 (liver, mitochondrial); glutaminase GA; glutaminase I; Glutaminase liver isoform, mitochondrial; L hL L-glutaminase; L hLGA; L-glutaminase; L-glutamine amidohydrolase; LGA; liver mitochondrial glutaminase; phosphate-activated glutaminase; phosphate-dependent glutaminase
Gene Aliases: A330074B06Rik; AI195532; GA; GLS; GLS2; hLGA; Kiaa4146; LGA
UniProt ID: (Human) Q9UI32, (Rat) P28492, (Mouse) Q571F8
Entrez Gene ID: (Human) 27165, (Rat) 192268, (Mouse) 216456
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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