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Invitrogen

GLT-1 Recombinant Rabbit Monoclonal Antibody (9H9L17)

Advanced Verification

1 Reference

View all (29) GLT-1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

Application

GLT-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

GLT-1 Antibody (701988) in ICC/IF

Immunofluorescence analysis was performed on fixed and permeabilized U-87 MG cells for detection of endogenous EAAT2 using Anti-EAAT2 Recombinant Rabbit Monoclonal Antibody (Product # 701988, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EAAT2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Produ... View More

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GLT-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells owing to their inherent genetic constitution. Relative expression of GLT-1 was observed in Mouse and Rat Brain and Cerebellum and not in Kidney and Lung tissues using GLT-1 Monoclonal Antibody (Product # 701988) in western blot. {RE}

Product Details

701988

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

9H9L17

Immunogen

Peptides corresponding to Human SLC1A2 (aa 5-20, 148-166)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2633044

Product Specific Information

This antibody is predicted to react with Monkey, Rabbit and Sheep

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhancing CDK4/6 inhibitor therapy for medulloblastoma using nanoparticle delivery and scRNA-seq-guided combination with sapanisertib.

Science advances

Lim C,Dismuke T,Malawsky D,Ramsey JD,Hwang D,Godfrey VL,Kabanov AV,Gershon TR,Sokolsky-Papkov M

701988 was used in Immunohistochemistry (Paraffin) to show the potential of nanoparticle formulation and scRNA-seq analysis of resistance to improve brain tumor treatment and identify POx-Palbo + Sapanisertib as effective combinatorial therapy for SHH medulloblastoma.

Fri Jan 28 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; Glutamate/aspartate transporter II; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2

Gene Aliases: 1700091C19Rik; 2900019G14Rik; AI159670; EAAT2; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2

UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596

Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482

Function(s)

L-glutamate transmembrane transporter activity high-affinity glutamate transmembrane transporter activity protein binding amino acid transmembrane transporter activity glutamate:sodium symporter activity symporter activity sodium:dicarboxylate symporter activity primary active transporter

Process(es)

amino acid transmembrane transport ion transport chemical synaptic transmission visual behavior response to wounding multicellular organism aging glutamate secretion telencephalon development adult behavior cellular response to extracellular stimulus multicellular organism growth response to drug response to amino acid positive regulation of glucose import L-glutamate import D-aspartate import L-glutamate transmembrane transport transport dicarboxylic acid transport nervous system development response to light stimulus L-glutamate transport sodium ion transmembrane transport neurotransmitter transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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