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GNA12 Antibody (PA5-120238) in WB
Western blot analysis of GNA12 in extracts of various cell lines. Samples were incubated with GNA12 Polyclonal antibody (Product # PA5-120238) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-120238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 285-381 of human GNA12 (NP_031379.2).
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913810
Product Specific Information
Positive test controls include: Mouse pancreas, Rat pancreas. The target is usually found in the following locations: Lipid-anchor, Membrane.
Immunogen sequence: LFFNVSIILF LNKMDLLVEK VKTVSIKKHF PDFRGDPHRL EDVQRYLVQC FDRKRRNRSK PLFHHFTTAI DTENVRFVFH AVKDTILQEN LKDIMLQ
Target Information
Guanine nucleotide-binding proteins (G proteins) are modulators or transducers in various transmembrane signaling systems. G proteins are composed of 3 subunits; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G alpha-12; G-protein subunit alpha-12; guanine nucleotide binding protein (G protein) alpha 12; guanine nucleotide binding protein, alpha 12; Guanine nucleotide-binding protein subunit alpha-12; WUGSC:H_GS165O14.2
Gene Aliases: AI414047; AI504261; Galpha12; gep; Gna-12; GNA12; NNX3; RMP
UniProt ID: (Human) Q03113, (Mouse) P27600, (Rat) Q63210
Entrez Gene ID: (Human) 2768, (Mouse) 14673, (Rat) 81663
in utero embryonic development
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
Rho protein signal transduction
blood coagulation
regulation of cell shape
regulation of fibroblast migration
cell differentiation
platelet activation
regulation of TOR signaling
regulation of proteasomal ubiquitin-dependent protein catabolic process
response to drug
embryonic digit morphogenesis
signal transduction
small GTPase mediated signal transduction
intracellular signal transduction
dopamine receptor signaling pathway
metabolic process
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