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Invitrogen

GNAO1 Polyclonal Antibody

Advanced Verification

2 Published Figures

3 References

View all (12) GNAO1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

GNAO1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

GNAO1 Antibody (PA5-30044) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of GNAO1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GNAO1 Polyclonal Antibody (Product # PA5-30044) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

GNAO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GNAO1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

GNAO1 Antibody in Immunoprecipitation (IP)

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GNAO1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of GNAO1 was observed in Mouse Brain in comparison to Mouse Lung, Mouse Kidney and Mouse Skeletal Muscle using GNAO1 Polyclonal Antibody product (Product # PA5-30044) in western blot. {RE}

Product Details

PA5-30044

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAO1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547518

Product Specific Information

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, mouse lung, rat brain, rat lung.

Predicted reactivity: Mouse (97%), Rat (98%), Xenopus laevis (98%), Chicken (98%), Rhesus Monkey (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an alpha-, beta- and gamma-subunit and is bound to GDP in the 'off' state. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; Galphas and Galphai bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNA Interference Effectors Selectively Silence the Pathogenic Variant GNAO1 c.607 G > A In Vitro.

Nucleic acid therapeutics

Klementieva NV,Lunev EA,Shmidt AA,Loseva EM,Savchenko IM,Svetlova EA,Galkin II,Polikarpova AV,Usachev EV,Vassilieva SG,Marina VI,Dzhenkova MA,Romanova AD,Agutin AV,Timakova AA,Reshetov DA,Egorova TV,Bardina MV

Published figure using GNAO1 polyclonal antibody (Product # PA5-30044) in Western Blot

Mon Apr 01 00:00:00 UTC 2024

CRISPR/Cas9-generated mouse model with humanizing single-base substitution in the Gnao1 for safety studies of RNA therapeutics.

Frontiers in genome editing

Polikarpova AV,Egorova TV,Lunev EA,Tsitrina AA,Vassilieva SG,Savchenko IM,Silaeva YY,Deykin AV,Bardina MV

Published figure using GNAO1 polyclonal antibody (Product # PA5-30044) in Western Blot

Fri Apr 21 00:00:00 UTC 2023

Customizing 3D Structures of Vertically Aligned Carbon Nanotubes to Direct Neural Stem Cell Differentiation.

Advanced healthcare materials

Nascimento L,Fernandes C,Silva RM,Semitela Â,de Sousa BM,Marques PAAP,Vieira SI,Silva RF,Barroca N,Gonçalves G

PA5-30044 was used in Western Blot to provide a new avenue for the construction of electroresponsive scaffolds based on CNTs for neural tissue engineering.

Sun Oct 01 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: DKFZp686O0962; G alpha o; GNAO 1; Go alpha; GO2-q chimeric G-protein; GTP-binding protein alpha o; GTP-binding protein alpha-o subunit; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide binding protein, alpha O polypeptide 1; Guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding regulatory protein 2

Gene Aliases: alphaO; AW050213; EIEE17; G-ALPHA-o; Galphao; Gna0; GNAO; GNAO1; HLA-DQB1; RATBPGTPC

UniProt ID: (Human) P09471, (Rat) P59215, (Mouse) P18872

Entrez Gene ID: (Human) 2775, (Rat) 50664, (Mouse) 14681

Function(s)

GTPase activity signal transducer activity GTP binding G-protein beta/gamma-subunit complex binding G-protein coupled serotonin receptor binding mu-type opioid receptor binding GTPase activating protein binding metal ion binding corticotropin-releasing hormone receptor 1 binding protein binding GDP binding protein complex binding nucleotide binding guanyl nucleotide binding heterotrimeric G-protein

Process(es)

protein folding muscle contraction adenylate cyclase-modulating G-protein coupled receptor signaling pathway dopamine receptor signaling pathway Wnt signaling pathway, calcium modulating pathway aging locomotory behavior regulation of heart contraction forebrain development neuron projection development response to cytokine response to drug response to hydrogen peroxide response to morphine positive regulation of GTPase activity negative regulation of calcium ion transport G-protein coupled receptor signaling pathway metabolic process cellular process response to organonitrogen compound response to organic cyclic compound signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GNAO1 Polyclonal Antibody

GNAO1 Antibody (PA5-30044) in ICC/IF

Product Details

PA5-30044

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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GNAO1 Polyclonal Antibody

GNAO1 Antibody (PA5-30044) in ICC/IF

Product Details

PA5-30044

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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