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Invitrogen

GNAS (R201C Mutant) Polyclonal Antibody

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)

Application

GNAS (R201C Mutant) Antibody in Western Blot (WB)

FIGURE: 1 / 1

GNAS (R201C Mutant) Antibody (PA5-119556) in WB

Western blot analysis of GNAS (R201C Mutant) in Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg). Sample was separated with 10% SDS-PAGE, incubated with polyclonal GNAS (R201C Mutant) antibody (Product # PA5-119556) at a dilution of 1:10000, and followed by HRP-conjugated anti-rabbit IgG.

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Product Details

PA5-119556

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant R201C of mouse GNAS. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.52 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2913129

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Information

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, which is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus, and the antisense transcript, are both paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Additional transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2015]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5530400H20Rik; A930027G11Rik; Adenylate cyclase-stimulating G alpha protein; alpha stimulating) complex locus; Alternative gene product encoded by XL-exon; C130027O20Rik; Extra large alphas protein; G protein subunit alpha S; Galphas; GNAS (guanine nucleotide binding protein; Gnas1; Gnasxl; GPSA; Gs alpha subunit; Gs-alpha; Gsa; GSP; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1; guanine nucleotide regulatory protein; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; Nesp; NESP55; Nespl; neuroendocrine secretory protein; Neuroendocrine secretory protein 55; Oed-Sml; Oedsml; P1; P2; P3; PHP1A; PHP1B; POH; Protein ALEX; SCG6; secretogranin VI; stimulatory G-protein alpha subunit; XLalphas

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Gene Aliases: 5530400H20Rik; A930027G11Rik; AHO; C130027O20Rik; C20orf45; Galphas; GNAS; GNAS1; Gnasxl; GPSA; GSA; GSP; MNCb-5546; NESP; Nespl; Oed-Sml; Oedsml; P1; P2; P3; PHP1A; PHP1B; POH; SCG6; SgVI

UniProt ID: (Human) Q5JWF2, (Mouse) Q6R0H7

Entrez Gene ID: (Human) 2778, (Mouse) 14683

Function(s)

GTPase activity signal transducer activity insulin-like growth factor receptor binding protein binding GTP binding G-protein beta/gamma-subunit complex binding beta-2 adrenergic receptor binding D1 dopamine receptor binding mu-type opioid receptor binding ionotropic glutamate receptor binding metal ion binding corticotropin-releasing hormone receptor 1 binding nucleotide binding G-protein alpha-subunit binding guanyl nucleotide binding protein domain specific binding G-protein beta-subunit binding alpha-tubulin binding heterotrimeric G-protein

Process(es)

tissue homeostasis endochondral ossification energy reserve metabolic process DNA methylation adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-activating dopamine receptor signaling pathway sensory perception of chemical stimulus embryonic hindlimb morphogenesis multicellular organism growth post-embryonic body morphogenesis response to drug positive regulation of osteoblast differentiation positive regulation of osteoclast differentiation developmental growth embryonic cranial skeleton morphogenesis cognition cartilage development bone development hair follicle placode formation platelet aggregation genetic imprinting regulation of parathyroid hormone secretion skeletal system development positive regulation of protein phosphorylation regulation of transcription from RNA polymerase II promoter signal transduction G-protein coupled receptor signaling pathway post-embryonic development regulation of signal transduction positive regulation of sodium ion transport positive regulation of cAMP biosynthetic process negative regulation of renal sodium excretion negative regulation of multicellular organism growth positive regulation of GTPase activity skin development positive regulation of cAMP-mediated signaling negative regulation of blood pressure calcium ion homeostasis response to parathyroid hormone cellular response to prostaglandin E stimulus cellular response to catecholamine stimulus adenylate cyclase-activating adrenergic receptor signaling pathway

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